毛细管气相色谱法测定臭冷杉挥发油中柠檬烯含量  

Determination of limonene in volatile oil of Abies nephrolepis leaves by CGC

作　　者：王威[1] 赵志兰[2] 牛志多[1] 苗艳波[1] 闫喜英[1] 师海波[1] 张宪平[1] 

机构地区：[1]吉林省中医中药研究院,吉林长春130021 [2]吉林省药品检验所,吉林长春130062

出　　处：《中草药》2003年第7期605-606,共2页Chinese Traditional and Herbal Drugs

摘　　要：目的　建立臭冷杉挥发油中有效成分柠檬烯的毛细管气相色谱测定方法。方法　色谱条件为 SE- 5 4毛细管色谱柱 ,程序升温 ,分流比为 4 0∶ 1,FID检测器 ,内标为萘。结果　柠檬烯浓度在 0 .6 0 132～ 1.4 0 30 8mg/ m L 呈线性关系 (r=0 .9996 ) ,平均加样回收率为 99.6 2 % (RSD=2 .4 1% )。结论　该法简便 ,灵敏 ,易操作 。Object To develop a reliable method for determination of limonene in volatile oil of Abies nephrolepis (Trautv.) Maxim. leaves by capillary gas chromatography (CGC). Methods SE 54 quartz capillary column was used and the temperature of column was programmed. The splitting ratio was 40∶1 The FID was used as detector, and the naphthalene as internal standard. Results The standard curve was linear in the concentraion range of 0 601 32 — 1 403 08 mg/mL (r=0 999 6). The average recovery was 99 62% with RSD=2 41%. Conclusion The method can be used for quality assessment of A. nephrolepis leaves and it is rapid, sensitive, precise and has good reproducibility.

关 键 词：臭冷杉 挥发油 柠檬烯 毛细管气相色谱 

分 类 号：R286.02[医药卫生—中药学]