乙肝病毒抗原在肝组织的表达及意义  

Expression and Significance of Hepatitis B Virus Antigen in Liver Tissues

作　　者：王怡[1] 赵桂鸣[1] 张万增[1] 

机构地区：[1]天津市传染病医院,300192

出　　处：《天津医药》2003年第7期420-421,共2页Tianjin Medical Journal

摘　　要：目的 :了解乙肝病毒感染者肝组织中乙肝病毒(HBV)抗原表达与病理分型及HBV复制的关系。方法 :采用免疫组织化学二步法检测肝组织HBsAg和HBcAg ,用ELISA法检测血清中HBV标志物。结果 :(1)HBV感染者肝组织中HBsAg和HBcAg检出率分别为79.75%和73.01 %。(2)乙肝病毒携带者 (AsC)HBsAg表达以浆型为主 ,膜型检出率较低。 (3)HBcAg浆型表达在重度肝病变高于轻度肝病变 ,而核型表达则相反。除中、重度外 ,各组间比较均有显著性差异 (P<0.01)。 (4)肝组织HBsAg、HBcAg 表达在血清HBeAg阳性组与HBeAb阳性组和HBeAg 和HBeAb均阴性组间有显著性差异 (P<0.01)。结论 :浆型HBsAg和核型HBcAg 多见于病变相对静止的病例 ,而膜型HBsAg 和浆型HBcAg 与病变活动程度明显相关。在血清HBeAg与HBeAb均阴性时 ,HBV仍有可能进行复制。HBcAg 作为主要的靶抗原 。Objective:To investigate the expression and significance of hepatitis B virus(HBV)antigne in liver tissues.Methods:In163patients with HBV infcetion,the levels of HBsAg and HBcAg of liver sample were detected with two-step technique in immunohistochemistry and the serum markers of HBV were tested with ELISA.Results:(1)The positive rates of HBsAg and HbcAg of liver sample were79.75%and73.01%respecˉtively.(2)In these patients,especially the cases of AsC,HBsAg mainly expressed in cytoplasm and the positive rates of cytomembrance were lower.There was no positive expression in24cases of AsC.(3)The positive rate of cytoplasmic HBcAg in patients with severe chronic hepatitis was significantly higher than that in patients with milder chronic hepatitis,but cytonuclear HbcAg was just the opposite.There were notable differences between the moderate and severe groups and the AsC or milder group(P<0.01).(4)The positive rates of HBsAg and HBcAg of liver sample were83.90%and80.51%in serum HBeAg(+)group and were77.78%and55.56%in serum HBeAb(+)group respectively.Conclusion:The cytomembrance HBsAg and cytoplasmic HBcAg were closely correlated with HBV replication and the degree of liver damage.When serum HBeAg-HbeAb were both negative,HBsAg and HBcAg were still found in the liver tissues of partial patients.

关 键 词：乙型肝炎 免疫发病机理 肝组织 抗原表达 病理分型 HBV复制 

分 类 号：R512.62[医药卫生—内科学]