高效毛细管电泳-安培检测法用于芦丁水解常数的研究  

The Study of the Hydrolysis Rate Constants of Rutin with Capillary Electrophoresis-Amperimetric Detection

作　　者：张兰[1] 陈强[1] 陈国南[1] 方禹之[2] 

机构地区：[1]福州大学化学系,福州350002 [2]华东师范大学化学系,上海200062

出　　处：《分析化学》2003年第7期779-784,共6页Chinese Journal of Analytical Chemistry

基　　金：中国博士后基金;福建省自然科学基金 (No .F0 2 10 0 0 4);福州大学博士后科技发展基金 (No .XJBH 610 2 );福州大学人才发展基金(No .2 0 0 2-XQ-13 )资助项目

摘　　要：用高分辨率、高灵敏度的毛细管电泳 安培检测法对芦丁水解的速率常数进行了研究 ,建立了芦丁及其水解产物槲皮素的定量分析方法。在优化电泳条件下 ,该方法能同时检测芦丁和槲皮素的峰电流随水解反应的进行而发生的变化。根据芦丁随着水解时间的不同而发生的浓度变化 ,计算求得水解的速率常数 ,并总结了温度对速率常数影响的规律。将方法用于芦丁和槲皮素的检测 ,芦丁与槲皮素的检测限分别为 0 .6g L和 6 .2mg L ;RSD(n =7)分别为 2 .36 %和 3.12 %。用于槐米中芦丁检测的回收率为 97.6 %。分析结果表明 ,此法用于测定芦丁水解常数简便、直观 ,用于芦丁和槲皮素的检测可靠性和重现性均很好。The concentration of rutin and its hydrolysis products were directly minitored by capillary electrophoresis-amperometric detection due to its high resolution and high sensitivity of detection. The hydrolysis rate constants of rutin can be obtained at different temperatures in 10% HCl. At 65 degreesC, 80 degreesC and 95 degreesC, the measured rate constants of rutin hydrolysis were 5.66 x 10(-3) min(-1), 7.19 x 10(-3) min(-1) and 8.51 x 10(-3) min(-1), respectively. The method was intuitionistic and convenient. The approach can also be used for separation and determination of the rutin and quercetin. Under the optimum conditions, the detection limit was 0.6 and 6.2 mg/L for rutin and quercetin, respectively. This method was successfully applied to the analysis of traditional medicine Huaimi sample with average recoveries of 97.6%.

关 键 词：毛细管电泳 安培检测法 芦丁 水解常数 速率常数 槲皮素 槐米 植物药 

分 类 号：O657.1[理学—分析化学] O658.9[理学—化学]