Spectral and Electrochemical Investigation of Intercalations of Adrenaline and CT-DNA  

作　　者：郑赛晶 林祥钦 

机构地区：[1]Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China

出　　处：《Chinese Journal of Chemistry》2003年第7期767-771,共5页中国化学（英文版）

基　　金：Project supported by the Live Science Research Base of University of Science and Technology of China(Grant No.KY1212)and the Key project of University of Science and Technology of China(Grant No.KY2216).

摘　　要：A strong interaction between double stranded calf-thymus DNA (ds-DNA) and adrenaline in solution, but no interaction between single stranded calf-thymus DNA (ss-DNA) and adrenaline was observed by the use of UV-visible spectroscopy and voltammetric techniques. It is suggested that the interaction leads to an intercalation of adrenaline molecules into the groove of ds-DNA and the formation of ds-DNA (adrenaline )?complex. The binding site size of the interaction of adrenaline with CT-DNA in nucleotide phosphate [NP] has been determined as 25. The interaction of different concentration a-drenaline with DNA modified GCE shows that the DNA modified GCE can be a good tool to detect lower concentration a-drenaline.A strong interaction between double stranded calf-thymus DNA (ds-DNA) and adrenaline in solution, but no interaction between single stranded calf-thymus DNA (ss-DNA) and adrenaline was observed by the use of UV-visible spectroscopy and voltammetric techniques. It is suggested that the interaction leads to an intercalation of adrenaline molecules into the groove of ds-DNA and the formation of ds-DNA (adrenaline )?complex. The binding site size of the interaction of adrenaline with CT-DNA in nucleotide phosphate [NP] has been determined as 25. The interaction of different concentration a-drenaline with DNA modified GCE shows that the DNA modified GCE can be a good tool to detect lower concentration a-drenaline.

关 键 词：DNA ADRENALINE UV-visible spectroscopy cyclic voltammetry differential pulse voltammetry 

分 类 号：O657.3[理学—分析化学] O657.1[理学—化学]