血管紧张素受体的表达在地氟醚预处理心肺转流前后的变化  

Gene expression of myocardial angiotensin receptor subtypes induced in patients under desflurane preconditioning before and after cardiac pulmonary bypass

作　　者：杨跃武[1] 杭燕南[1] 孙大金[1] 

机构地区：[1]上海第二医科大学附属仁济医院麻醉科,200127

出　　处：《临床麻醉学杂志》2003年第7期392-394,共3页Journal of Clinical Anesthesiology

摘　　要：目的　研究血管紧张素受体Ⅰ型、Ⅱ型 (AT1、AT2 )在地氟醚预处理心肺转流 (CPB)前后的变化。方法　选择行瓣膜置换术病人 2 0例 ,随机分为两组 :D组CPB前吸入 6 %～ 9%地氟醚 ,持续时间不少于 30min ;F组以芬太尼为主行全凭静脉麻醉。分别于CPB前后取右心耳组织约5 0mg ,逆转录聚合酶链式反应 (Rt PCR)测其中AT1、AT2受体的基因表达。 结果　两组病人在CPB前后AT1受体均有表达 ,组内比较无显著差异 (P >0 0 5 ) ,组间比较差异显著 ,即F组高于D组 (P<0 0 1 ,P <0 0 5 ) ;CPB前两组病人的AT2受体均无表达 ,CPB后均见AT2受体表达 ,以D组显著高于F组 (P <0 0 1 )。结论　血管紧张素受体参与了地氟醚预处理对缺血Objective To investigate the gene expression of angiotensin receptor subtypes(AT1,AT2)induced by desflurane preconditioning before and after cardiac pulmonary bypass(CPB).Methods Twenty patients undergoing cardiac valve replacement were divided rendomly into two groups:D group patients inhaled 6% 9% desflurane continuously at least for 30min before CPB;F group patients received fentanyl mainly for TIVA.50mg right atrial appendages were taken before and after CPB.Expressions of AT1,AT2 receptors were measured by reverse transcription polymerase chain reaction(Rt PCR).Results The expression of AT1 receptor was found pre and post CPB in both groups,but there were no significant differences in the respective group.The expression in D group was lowere than that in F group( P< 0 01, P< 0 05);before CPB,AT2 had no expression,but after CPB,AT2 expression was found in the two groups,which was higher in the D group than that in the fentanyl group( P< 0 01).Conclusion Angiotensin receptors regulate the effect of desflurane preconditoning for CPB patients. [

关 键 词：血管紧张素受体 表达 地氟醚 预处理 心肺转流 麻醉 

分 类 号：R614[医药卫生—麻醉学]