大鼠小体积肝移植早期移植物巨噬细胞炎性蛋白-2的表达  

Early expression of macrophage inflammatory protein-2 in isografts of the rat with small-for-size liver transplantation

作　　者：梁廷波[1] 徐世国[1] 郑树森[1] 谢海洋[1] 蒋国平[1] 秦运升[1] 

机构地区：[1]浙江大学医学院附属第一医院肝胆胰外科暨器官移植中心,杭州310003

出　　处：《中华普通外科杂志》2003年第7期418-420,共3页Chinese Journal of General Surgery

基　　金：浙江省卫生厅资助项目 (编号 2 0 0 2A0 41) ;浙江省科技厅资助项目 ( 2 0 0 3C3 40 0 6)

摘　　要：目的　探讨大鼠小体积肝移植术后早期移植物内巨噬细胞炎性蛋白 2 (MIP 2 )的表达及其意义。方法　建立大鼠全肝 ,5 0 %及 30 %小体积肝移植模型 ,分别于术后 0 5 ,2 ,6和 2 4h处死大鼠 ,同时以假手术组作为对照 ,通过ELISA法检测血浆TNF α含量 ,半定量RT PCR方法检测肝组织内MIP 2mRNA的表达 ,同时进行常规病理学检查。结果　 (1)血浆TNF α高峰出现于术后 2h ,全肝移植组在 2h显著高于 30 %肝移植组 (377± 12 6 ,81± 2 3,t=2 5 5 ,P <0 0 5 ) ;(2 )MIP 2mRNA的表达 :移植组的表达显著高于假手术组 (P <0 0 1) ,均于 2h达到高峰 ,5 0 %肝移植组和 30 %肝移植组在 2 4h时点显著高于全肝移植组 (P <0 0 1) ;(3)病理学检查提示全肝移植组各时点肝组织学结构基本正常 ,5 0 %肝移植组于术后 2 4h时点可见肝窦扩张 ,而 30 %肝移植组于术后 6h时点可见肝窦扩张 ,2 4h更严重 ,并可见肝细胞胞浆内空泡形成。结论　小体积肝移植早期移植物内存在MIP 2mRNA表达升高 ,这可能与肝移植后肝损伤的发生有关 ,其表达高低与移植肝体积相关。ObjectiveTo investigate the expression of macrophage inflammatory protein 2 (MIP 2) in isografts of the rat with small for size liver transplantation. MethodsThe animal models of whole graft, 50% size and 30% size graft liver transplantation were established. The animals were sacrificed at 30 minute, 2 , 6 and 24 hour time points to harvest the graft specimens and blood samples. The level of serum TNF α was measured by ELISA and MIP 2 gene expression level was detected by semi quantitive reverse transcribed polymerase chain reaction (RT PCR). Results (1)The level of serum TNF α of whole graft group was significantly higher than that of 30% size group at 2 hour time point( P < 0 05); (2) The MIP 2 mRNA expression of transplantation groups was significantly higher than that of sham operation group ( P <0 01) and the expression of whole graft group was significantly lower than that of 50% size group and 30% size group at 24 hour time point ( P <0 01). (3)The morphological structure was normal at all time points in whole graft transplantation group. Liver sinusoid dilation was found at 24 hour time point in 50% size group and at 6 hour time point and more severe at 24 hour time point in 30% size group. Vacuolization formation in cell cytoplasm was detected at 24 hour time point in 30% size group. ConclusionsThe up regulation of MIP 2 mRNA expression in isografts may be responsible for the graft injury after small for size liver transplantation and their expression was correlated with the size of grafts.

关 键 词：大鼠 肝移植 早期 移植物 巨噬细胞 炎性蛋白-2 表达 

分 类 号：R657.3[医药卫生—外科学]