羟基磷灰石/聚DL-乳酸复合内固定材料对成骨细胞活性影响的体外研究  

AN IN VITRO STUDY OF HYDROXYAPATITE/POLY-DL-LACTIDE COMPOSITE INTERNAL FIXATION ON OSTEOBLASTS

作　　者：陆博[1] 郑启新[2] 

机构地区：[1]河北医科大学第三医院关节骨科 [2]华中科技大学附属协和医院骨科,武汉430022

出　　处：《中国生物医学工程学报》2003年第4期350-357,共8页Chinese Journal of Biomedical Engineering

基　　金：国家科委重点科技攻关项目 ( 9692 0 2 0 11);武汉市重点科技攻关项目 ( 960 0 2 0 5 6G);湖北省自然科学基金资助项目 ( 99J0 5 3 )

摘　　要：目的 :与单纯聚DL 乳酸 (PDLLA)材料比较研究羟基磷灰石 /聚DL 乳酸 (HA/PDLLA)复合内固定材料的降解产物对成骨细胞增殖和分化的影响。方法 :检测HA/PDLLA材料和PDLLA材料体外降解过程中降解液 pH值、钙离子、无机磷浓度的变化 ;通过四甲基偶氮唑盐 (MTT)酶反应比色法检测成骨细胞在材料降解产物作用下的增殖情况 ,通过检测碱性磷酸酶 (ALP)活性了解其分化情况 ,扫描电镜 (SEM)观察成骨细胞在材料直接作用下的形态学表现。结果 :体外自然降解时 ,HA/PDLLA材料降解液 pH值下降较PDLLA材料慢 ,HA/PDLLA材料降解时有钙磷成分溶出。与对照组比较 ,HA/PDLLA材料所设全部七个浓度的浸提液及PDLLA材料从 1∶2 5 6到 1∶16这四个浓度的浸提液对成骨细胞增殖均无抑制 (P >0 .0 5 ) ,PDLLA材料 1∶4及 1∶1两个浓度的浸提液抑制了成骨细胞增殖 (P <0 .0 5及P <0 .0 1)。作用 1d和 4d后的成骨细胞ALP活性在HA/PDLLA材料组与同期对照组相比均无显著性差异(P >0 .0 5 ) ,在PDLLA材料组与同期对照组及HA/PDLLA材料组相比均有显著性升高 (P <0 .0 1)。HA/PDLLA材料表面的成骨细胞呈纺锤形及延展为多角状 ;PDLLA材料表面的成骨细胞呈球形及延展不充分 ,且数量较少 ;HA/PDL LA材料表面可见HA颗粒。结论 :HA/PDLLA材料降?Objective: To compare the influence of the degradation products of Hydroxyapatite/Poly-DL-lactide(HA/PDLLA) composite on proliferation and differentiation of osteoblasts, with single Poly-DL-lactide (PDLLA); and to provide scientific basis for use of implanting devices made of HA/PDLLA composite. Method: HA/PDLLA and PDLLA immersed were into 37°C saline, and at different moments the pH and concentrations of calcium and phosphate ions of the degrading solution measured. Extracting solutions of these two kinds of materials were sterilized under 70°C for 24 hours. A series of solutions of different concentrations were added to osteoblasts cultured in vitro. The proliferation of osteoblasts was evaluated by MTT method, and the differentiation of osteoblasts was evaluated by ALP activity. SEM was conducted to observe the osteoblasts' morphologic manifestation on the material surface. Result: The pH of HA/PDLLA and PDLLA solutions decreased with time, being decreased more rapidly in HA/PDLLA group than in PDLLA groups. Calcium and phosphate ions were detectable in the degrading solutions of HA/PDLLA. The results of MTT method showed no significant difference detectable between HA/ PDLLA groups (for all concentrations) and the control group (P > 0.05), and no significant difference detectable between PDLLA groups (concentrations from 1: 256 to 1: 16) and control group (P > 0.05); while the proliferation of ostsoblasts in PDLLA group (of concentration 1: 4 was inhibited significantly compared with control group (P > 0.05), and the PDLLA group of concentration 1: 1 was inhibited more significantly compared with control group (P 0.05); in contrast, in PDLLA group the ALP activities were significantly higher than the control group either at the first day or at the fourth day (P < 0.01). The SEM result showed that the morphologic manifestation of the osteoblasts on the HA/PDLLA surface was more typical with spindle-like and polygonal morphology than those on the PDLLA surface. HA granules could be found in the SEM photos.

关 键 词：可吸收材料 成骨细胞 羟基磷灰石 聚DL乳酸 体外 

分 类 号：R318.08[医药卫生—生物医学工程]