两种生防促生菌与青霉菌灭活菌丝体协同诱导烟草抗病效果研究  

Study on the Resistance of Tobacco Induced by Two Kinds of Bio-control Bacterium Cooperate with Dry Mycelium of Penicillium chrysogenum

作　　者：吴剑[1,2] 张廷金[3] 李祥军 何军 王继明 郑元仙 闫春丽 尹忠仁 陈穗云[2] 

机构地区：[1]云南省烟草公司临沧市公司,云南临沧677000 [2]云南大学生命科学学院,云南昆明650091 [3]昆明保腾生化技术有限公司,云南昆明650106

出　　处：《青海师范大学学报（自然科学版）》2015年第3期34-39,共6页Journal of Qinghai Normal University(Natural Science Edition)

基　　金：云南省烟草公司科技计划项目(合同编号:2013YN36)

摘　　要：本试验开展了青霉菌灭活菌丝体(dry mycelium of Penicillium chrysogenum,DMP)与木霉菌菌剂和芽孢杆菌菌剂在临沧烤烟生产上的协同应用研究.结果表明,移栽时塘施DMP,团棵期和打顶前再喷施木霉菌菌剂,旺长期再灌施芽孢杆菌菌剂的处理现蕾期和成熟期最大腰叶的长、宽和面积均显著大于常规防治处理、空白对照和仅施DMP的处理;该处理现蕾期对烟草普通花叶病的防效为80.12%,高于常规防治25.30%,高于仅施DMP的处理25.90%;对烟草赤星病的防效为62.24%,高于常规防治5.83%,高于仅施DMP的处理13.29%;对烟草黑胫病的防效为77.42%,高于常规防治15.32%,高于仅施DMP的处理26.61%;该处理的产量为1886.70kg/hm2,比空白对照增产196.80kg/hm2,比常规防治处理增产96.60kg/hm2;该处理的产值为49450.41元/hm2,比空白对照增值6493.15元/hm2,比常规防治处理增值3104.72元/hm2.An application research of dry mycelium of Penicillium chrysogenum (DMP) cooperate with two kinds of bio-control bacterium was carried out in Lincang Tobacco-growing area. The results indicated that,employ DM P in the plant hole before tobacco seedling transplant,then spray trichoderma agent in re-settling stage and before topping,and root-irrigate bacillus agent in the period of vigorous grow th can sig-nificantly improve the length,the width and the area the biggest waist leaf in squaring period and mature period compare with conventional control,blank control and deal with DMP only. The relative control effect of the above mentioned treatment to tobacco mosaic virus amounted to 80.12%,higher than 25.30%of conventional control,higher than 25.90% of treatment deal with DMP. The relative control effect of the above mentioned treatment to tobacco blown spot amounted to 62.24%,higher than 5.83% of the con-ventional control,higher than 13.29% of the treatment deal with DMP only. The relative control effect of the above mentioned treatment to tobacco black shank amounted to 77.42%,15.32 points higher than the conventional control,higher than 26.61% of the treatment deal with DMP. The yield of the above men-tioned treatment is 1886.70kg/hm2,196.80kg/hm2 higher than the blank control,96.60kg/hm2 higher than conventional control. The production value of tobacco leaf reached 49450.41 Yuan per hectare, 6493.15 Yuan per hectare higher than the blank control,3104.72 Yuan per hectare higher than conven-tional control.

关 键 词：青霉菌灭活菌丝体 芽孢杆菌 木霉菌 烟草普通花叶病 烟草赤星病 烟草黑胫病 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]