选择性脑超深低温技术对猴颈动脉血流阻断时限的研究  

The duration of selective cerebral ultraprofound hypothermia following both carotid arteries clip in mon key

作　　者：江基尧[1] 徐蔚[2] 杨朋范 朱诚[4] 高永军[2] 高国一[4] 孙亮[2] 

机构地区：[1]上海第二医科大学仁济医院,20001 [2]昆明医学院二附院神经外科 [3]福州军区总医院 [4]第二军医大学长征医院

出　　处：《中华神经外科杂志》2003年第4期304-306,共3页Chinese Journal of Neurosurgery

基　　金：上海市科委重点项目基金(NO.994119089);教育部科学技术研究重点项目(NO.02141);云南省自然科学基金资助项目(NO.2002C0052)

摘　　要：目的研究选择性脑超深低温断血流对猴神经功能、神经元超微结构和对全身主要器官功能的影响。方法通过一侧颈内动脉冷灌注,同侧颈内静脉回流,阻断其他颈部血管,建立选择性脑局部体外循环通路,60～80min后恢复脑血流,实验动物自然复苏。脑血流阻断前后监测各主要脏器的生化指标变化、观察脑神经功能变化、定时处死动物行全脑电镜检查。结果5只猴都实现了脑选择性深低温,最低脑温达15.5±2.29℃,中心体温30.4±0.96℃。降温过程中及术后猴生命体征平稳;4只长时间存活(1～40周),术后神经功能评分无异常,神经元超微结构无明显异常;各主要脏器生化指标无显著变化。结论脑选择性深低温可明显提高脑对缺血缺氧的耐受性,对全身主要器官无明显损害。为临床使用该技术提供了可靠的实验依据。Objective To determine the effects of selective cerebral profo un d hypothermia on nerve functions and ultrastructure as well as systemic organs i n monkeys.Methods Selective cerebral circulation was established by perfusion of cooling Linger's liquid through right internal carotid artery and flow out of left jugular vein with clip of all other carotid arteries and jugular veins. Cerebral blood flow recovered after60～80minutes of cerebral ischemia and mo nkey came back naturally.The cerebral functions and ultrastructures as well as systemic organ were examined.Results Brain temperature reached15.5±2.2 9℃while the body temperature maintained30.4±0.96℃during selective ce rebral cooling linger's liquid perfusion in5monkeys.Four of five monkeys surv ived after carotid clip-reperfusion model.Neurological functions and cerebral ultrastracture as well as systemic organs kept normal at1～40weeks follow-up.Conclusion Selective cerebralultraprofound hypothermia is safety and may pro vide marked protective effects and safety during cerebral ischemia.

关 键 词：选择性脑超深低温技术 猴 颈动脉血流阻断时限 研究 神经功能 神经元超微结构 

分 类 号：R651.1[医药卫生—外科学]