掺铈纳米二氧化铅修饰电极色谱电化学用于单胺类神经递质的研究  

Detection of Monoamine Transmitters Using High Performance Liquid Chromatography with Nanocrystalline Ce-doped Lead Dioxide Deposited Film Chemically Modified Electrode

作　　者：张文[1] 谢云峰[1] 艾仕云[1] 万芳利[1] 王坚[2] 金利通[1] 

机构地区：[1]华东师范大学化学系,上海200062 [2]复旦大学附属华山医院神经内科,上海200400

出　　处：《化学学报》2003年第7期1030-1035,共6页Acta Chimica Sinica

基　　金：国家自然科学基金(No.20175006);国家科技部基金(No.2001BA210A04);上海市科委(No.0114nm072)

摘　　要：研制了一种新型掺铈纳米二氧化铅修饰电极,用X射线衍射(XRD)和扫描隧道显微镜(STM)进行了表征.该修饰电极对单胺类神经递质及其代谢产物有良好的电催化作用,灵敏度高,稳定性和重现性好,将其作为电化学检测器与液相色谱和微渗析技术联用,测定了帕金森氏症病人脑脊液中单胺类神经递质及其代谢产物的含量.取得了满意的结果并初步探讨了帕金森氏症的致病机理.A novel nanocrystalline Ce-doped lead dioxide (PbO2) deposited film modified glassy carbon electrode was prepared. The modified electrode was characterized by X-ray diffractometer (XRD) and scanning tunneling microscope (STM). techniques. The nanocrystalline Ce-doped PbO2 deposited film modified electrode showed favorable electrocatalytic behavior toward the monoamine neurotransmitters with relatively high sensitivity and stability. Coupled to high-performance liquid chromatography ( HPLC) with microdialysis, the modified electrode was utilized for electrochemical detection (ED) of these compounds. The method was successfully applied to assess the contents of the monoamines and their metabolites in the Parkinsonian patient's cerebrospinal fluid ( CSF). The mechanism of Parkinsons disease was discussed elementarily.

关 键 词：掺铈纳米二氧化铅 修饰电极 色谱电化学 单胺类神经递质 帕金森氏症 脑脊液 

分 类 号：O657.1[理学—分析化学]