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机构地区:[1]深圳市中医院,广东深圳518033 [2]河南省中医院,河南郑州450003 [3]广州中医药大学,广东广州510407
出 处:《中国中医基础医学杂志》2003年第6期30-32,67,共4页JOURNAL OF BASIC CHINESE MEDICINE
摘 要:目的 :研究中药复方髓清丸对白血病细胞的作用。方法 :采用血清药理学方法体外培养观察人红白血病细胞系 (HEL)的增殖、凋亡、细胞周期、细胞形态等细胞学指标。结果 :活细胞百分率各含药血清组均较对照组低 ,MTT法测定髓清丸中剂量组和高剂量组OD值明显低于对照组 (p<0 0 1) ,中剂量组和高剂量组细胞毒活性均大于 2 0 %,高剂量含药血清组G1期DNA含量明显低于对照组 (P <0 0 5 ) ,中剂量组凋亡细胞百分率较高 (P <0 0 5 )。结论 :髓清丸能抑制HEL细胞的增殖 ,并有一定的诱导HEL细胞分化、凋亡的作用。To study the effect of Sui-Qing-Wan (SQW) on l ukemia cell.Methods: Human erythroleukemia cell line (HEL) was studied in Prolif eration,Apoptosis,cell proliferous cycle,Cytomorphology with serum-pharmacology methods.Results:The live cell percent,live cell absolute count and optical dens ity (OD) of MTT in three dose group were all lower than those in control group d istinctly ( P<0.05 ),cytotoxicity in the moderate group and the high dose gro up were all larger than 20%.DNA content of G 1 period in high dose group of SQW was higher than those in control group markedly ( P<0.05 ).It indicated that SQW could restrain malignant cell growth.Apoptosis peak was found in HEL cutured with YDQ moderate dose by FCM( P<0.05 ).Conclution:SQW can inhibite prolifer ation of HEL cell,induce differentiations and impell apoptosis.
关 键 词:髓清丸 HEL 血清药理学 白血病 细胞周期 细胞凋亡 细胞增殖
分 类 号:R273.37[医药卫生—中西医结合] R285.5[医药卫生—中医肿瘤科]
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