“十二井穴”针法对局灶性脑缺血大鼠TGF-β1的时效性影响  

Effect of Acupuncture of "Twelve Jing Points" on TGF-β1 Expression in Cerebral Focal Ischemia Rats

作　　者：郝晋东[1] 孟宏[1] 金智秀[1] 赵万标[1] 图娅[1] 

机构地区：[1]北京中医药大学针灸学院,北京100029

出　　处：《针刺研究》2003年第2期89-93,共5页Acupuncture Research

摘　　要：目的 :观察“十二井穴”针法对局灶性脑缺血大鼠TGF β1的时效性影响。方法 :采用光化学诱导Wistar大鼠一侧大脑造成局灶性脑缺血模型 ,施以“十二井穴”针法在造模后 1、3、6、1 2、2 4hr不同时间段开始进行针刺治疗 ,治疗 6天后处死大鼠 ,沿缺血中心制成冠状面切片 ,运用免疫组化染色观察各治疗组半暗带与对照组神经细胞TGF β1表达量的变化 ,并进行图像分析。结果 :各时段模型组TGF β1表达的总面积及 3、6、1 2、2 4hr各组的积分光度均比假手术及正常组明显升高 (P <0 0 5) ,针刺组 3、6、1 2、2 4hrTGF β1表达的总面积及 6、1 2、2 4hrTGF β1表达的积分光度均较同时段模型组明显升高 (P <0 0 5或P <0 0 1 )。结论Objective: To observe changes of transforming growth factor (TGF)-β1 expression in neurons of the brain after cerebral ischemia and acupuncture of the 'twelve Jing points' in the rats. Methods: 96 Wistar rats were randomized into model group (n=30), acupuncture group (n=30), sham-operation group (n=30) and control group (n=6), the former 3 groups were further evenly divided into 1, 3, 6, 12 and 24 hr subgroups. The rat focal cerebral ischemia model was made by photochemical method to block blood flow of the middle cerebral artery. The 'twelve Jing points'(LU-11, LI-1, PC-9, TE-1, HT-9, SI-1) were punctured with filiform needles, starting respectively at 1, 3, 6, 12, 24 hr after establishing ischemia models, once daily, 6 times altogether. 6 days later, the rats were put to death for taking the brain tissue sample (ischemic region) that was then cut into coronal slices. Immuno-histochemical staining was performed for displaying changes of TGF-β1 expression of the cerebral neurons in the penumbra region. Then image analysis was conducted. Results: In comparison with sham-operation and control groups, TGF-β1 expression area of model group increased significantly (P<0. 01) from 3 hr on after cerebral ischemia. Compared with model group, TGF-β1 expression area of acupuncture group increased further significantly from 3 hr on after cerebral ischemia (P< 0.05,0.01). The integral luminosity value of various groups presented a similar tendency in spite of appearing later (6 hr after cerebral ischemia) in the significant difference. Morphological observation indicated that TGF-β1 expression came from microgliocytes and phagocytes. Conclusion: The results prove that acupuncture can significantly upregulate TGF-β1 expression, which maybe contribute to the protection effect of acupuncture on ischemic cerebral neurons.

关 键 词：局灶性脑缺血 转化生长因子-Β1 针刺疗法 “十二井穴”针法 半暗带神经元 

分 类 号：R277.733.1[医药卫生—中医学] R246.6