电针镇痛时炎症痛大鼠PAG部位I型白细胞介素-1受体mRNA表达的变化  

Changes of Expression of IL-1 Receptor-I mRNA in Rat Periaqueductal Gray after Peripheral Inflammation and Electroacupuncture Analgesia

作　　者：姬广臣[1] 俞瑾[1] 董志强[1] 吴根诚[1] 

机构地区：[1]复旦大学医学院针刺原理研究所

出　　处：《针刺研究》2003年第2期111-114,140,共5页Acupuncture Research

基　　金：国家自然科学基金 (No 3 9970 92 5 );上海市针灸经络研究中心项目 ( 0 2DZ19- 15 0 - 0 1);上海市中医药重大项目基金资助(No 2 0 0 0 - 3 )

摘　　要：目的 :本实验观察炎症痛和针刺镇痛时 ,大鼠中脑导水管周围灰质 (PAG)部位I型白细胞介素 1受体基因 (IL 1RImRNA)表达的变化。方法 :实验分正常组、炎症痛组、假电针组、电针组。正常对照组大鼠脚掌注射生理盐水 ,炎症痛各组大鼠脚掌注射 2 %角叉菜胶 ( 1 0 0 μL)造成炎症痛模型。在角叉菜胶注射 3hr后大鼠处于一种稳定的痛敏状态。电针组大鼠于造模前电针将致炎侧“足三里”和“昆仑”穴 3 0min ,假电针组大鼠给予同样处理但不通电。实验采用原位杂交技术 ,观察脚掌注射角叉菜胶 3hr后大鼠中脑导水管周围灰质 (PAG)部位IL 1RImRNA表达的变化及针刺对其的影响。结果 :致炎后 3hr大鼠痛阈显著降低 ,大鼠PAG部位的IL 1RImRNA表达显著增加 ,假电针组大鼠该部位的IL 1RImRNA表达与炎症痛组相比无显著变化 ,而电针组大鼠该部位的IL 1RImRNA阳性细胞数与假电针组相比显著减少。结论 :炎症痛可引起大鼠PAG部位的IL 1RImRNA表达增加 ,而电针镇痛则抑制炎症痛引起的大鼠PAG部位ILObjective: The aim of the present study was to observe the changes of expression of IL-1R(receptor)-I mRNA in rat periaqueductal gray after peripheral inflammation and electroacupuncture (EA) analgesia. Methods: Twenty-four SD rats were evenly divided into normal group, inflammation group, sham EA group and EA group. Rats of normal group received an intraplantar injection of 100 μL normal saline, and those of inflammation group, sham EA group or EA group received an intraplantar injection of 100 μL carrageenan (2%, 100 μL). EA stimulation (4～60 Hz,1～2～3 mA, every 10 min for each current strength) was applied to unilateral 'Zusanli' (ST 36) and 'Kunlun' (BL 60) 3 hr after intraplantar injection of carrageenan. Sham EA was performed in the same way as EA except no EA was given. The expression of IL-1RI mRNA in rat periaqueductal gray following peripheral carrageenan inflammation was measured using in situ hybridization technique. The paw withdraw latency (PWL) was detected before and after different treatment. Results: Three hours after injection of carrageenan, PWL values of normal control, inflammation, sham EA and EA groups were 13.34±2.11 sec, 2.03±0.16 sec, 2.27±0.18 sec and 5.16±1.26 sec respectively; while compared with inflammation and sham EA groups, PWL of EA group increased significantly (P<0.05). Following carrageenan inflammation, the expression of IL-1RI mRNA in rat periaqueductal gray was significantly increased (positive reaction cells: 60.86±7.38, vs control group: 13.67±2.74, P< 0.001). Sham EA did not influence the expression of IL-1RI mRNA (61.45±6.49 positive cells), but EA could significantly inhibit the expression of IL-1RI mRNA (37.08±6.36 positive cells, P<0.05, vs inflammation and sham EA groups) in the same central region in carrageenan inflammatory rat. Conclusion: Carrageenan inflammation increases the expression of IL-1RI mRNA in rat periaqueductal gray, and EA analgesia could significantly inhibit the expression of IL-1RI mRNA in carrageenan inflammatory rats.

关 键 词：炎症痛 电针 Ⅰ型白细胞介素-1受体基因 脑导水管周围灰质部位 足三里 昆仑穴 

分 类 号：R246.2[医药卫生—针灸推拿学] R245.97[医药卫生—中医临床基础]