复方黄芩片质量标准的研究  

A Study on Quality Standard of Compound Huangqin Tablets

作　　者：曾元儿[1] 卢文彪[1] 钟镜金[1] 

机构地区：[1]广州中医药大学中药学院,广州510405

出　　处：《中药新药与临床药理》2003年第4期260-262,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立复方黄芩片的质量控制标准。方法采用薄层色谱法对复方黄芩片中的黄芩、虎杖、功劳木等进行了定性鉴别。采用HPLC法对制剂中的黄芩苷进行了含量测定。流动相:甲醇-0.4%磷酸(50∶50),流速:1.4mL/min;柱温:40℃;检测波长:278nrn。结果鉴别项下阴性对照无干扰,专属性强;黄芩苷在0.12～0.72μg范围内有良好线性关系(r=0.9996),平均回收率为97.17%,RSD=1.78%(n=5)。结论该方法可为复方黄芩片的质量评价提供科学依据。Objective To establish the standard for the quality control of Compound Huangqin Tablets. Methods The identification of Radix Scutellariae,Rhizoma Polygoni Cuspidati and Caulis Mahoniae in compound Huangqin tablets were carried out by TLC.The content of baicalin in the preparation was determined by HPLC.Chromatocolumn was adopted,methanol-0.4 %H3PO4(50 ∶50) used as the mobile phase,flowrate 1.4mL/min,column temperature at 40 ℃and detection wavelength 278 nm . Results The TLC spots were highly clear without the interference of negative control and were reproductive. The calibration curve for baicalin was linear (r=0.9996) in the range of 0.12～0.72 μg and the mean recovery was 97.17 %and RSD=1.78 %(n=5). Conclusion This method can be used for the quality control of Compound Huangqin Tablets.

关 键 词：@复方黄芩片/化学 黄芩/化学 虎杖/化学 功劳木/化学 黄芩苷/分析 色谱法 薄层 色谱法 高压液相 

分 类 号：R284.1[医药卫生—中药学]