银杏外种皮多糖对人胃癌细胞凋亡及其凋亡诱导基因表达的影响  

Influence of ginkgo biloba exocarp polysaccharides( GBEP) on apoptosis and expression of inducing apoptosis' gene in human gastriccancer SGC-7901 Cell

作　　者：许爱华[1] 陈华圣[1] 孙步蟾[1] 顾玉兰[1] 

机构地区：[1]扬州大学医学院,江苏扬州225001

出　　处：《中药药理与临床》2003年第3期11-13,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：江苏省科技厅社会发展项目基金资助 (NOBS2 0 0 0 0 86);江苏省教育厅自然科学基金资助 (NO 99KJD360 0 0 1 )

摘　　要：目的 :研究银杏外种皮多糖对人胃癌细胞凋亡及其凋亡诱导基因表达的影响。方法 :应用体外培养方法和流式细胞技术 ,检测人胃癌SGC 790 1细胞的凋亡率及其凋亡诱导基因bax的阳性蛋白标记率。结果 :银杏外种皮多糖 40～ 160 μg/ml可诱导人胃癌SGC 790 1细胞的凋亡 ,在培养 2 4h～ 72h期间 ,具有量效关系和时效关系。银杏外种皮多糖 40～ 160 μg/ml也同时促进bax基因的表达。结论 :银杏外种皮多糖的抗肿瘤作用机制之一可能与其诱导癌细胞凋亡有关 ,其诱导癌细胞凋亡的作用机制之一可能与其影响bax基因的表达有关。Objective: To study the Influence of ginkgo biloba exocarp polysaccharides( GBEP) on apoptosis and expression of inducing apoptosis' gene in human gastriccancer SGC 7901. Method: Using techniques of cell culture and flow cytometry in vitro, to measured the rate of apoptosis and positive sign of bax gene protein of human gastriccancer SGC 7901 cells. Result: GBEP (40～160μg/ml, 24h～72h) may induce apoptosis and exhibited dose and time effect on SGC 7901 cells. At the same time, GBEP (40～160μg/ml, 48h) also increased the expression of bax gene of SGC 7901 cells. Conclusion: One of antitumor action mechanisms of GBEP might be it induced tumor cells' apoptosis . one of action mechanisms of GBEP that induced tumor cells to apoptosis has some relationship with the changes of the bax gene.

关 键 词：银杏外种皮多糖 胃癌 细胞凋亡 诱导基因 表达 影响 

分 类 号：R273[医药卫生—中西医结合]