HPCE-FPS技术对吴茱萸质量的研究  

Quality study on evodiamine of Fructus Evodia by HPCE-FPS

作　　者：李琴韵[1] 王智华[1] 洪筱坤[1] 

机构地区：[1]上海中医药大学,上海200032

出　　处：《中成药》2003年第7期570-573,共4页Chinese Traditional Patent Medicine

摘　　要：目的 :采用HPCE CZE法对中国药典收载的 3个不同品种的 14个吴茱萸样品 (不同产地 )中所含吴茱萸碱进行含量测定 ,同时还采用两种波长下 ,建立了吴茱萸样品的高效毛细管电泳色谱 指纹谱 (HPCE FPS)。方法 :采用HP3D全自动毛细管电泳仪对 14个吴茱萸样品中所含的吴茱萸碱在波长为 2 5 4nm处进行定量测定 ,同时在 196nm ,2 5 4nm处对上述样品中的蛋白质类物质进行指纹谱的分析。结果 :通过方法学的考察 ,吴茱萸碱在 1.94 4～ 7.776 μg·mL-1范围内呈良好的线形关系 ,其回归方程为 y =188.7x - 32 .1,相关系数r =0 .9974 ,平均回收率为 10 1.3% (n =3) ,建立了不同品种吴茱萸的蛋白指纹谱。结论 :实验建立蛋白类物质的指纹谱 ,能将 3种不同品种的吴茱萸明显地区分 ;能揭示了其内在质量 ,研究为中药的质量评估研究提供了新的思路和切实可行的方法。Objective: A HPCE capillary zone eletrophoretic method for the determination of evodiamine in Fructus Evodia was established. Similarly, areliable HPCE-FPS (fingerprint spectrum) was applied to compare quality of Fructus Evodia to 14 samples of 3 species from different areas. Methods: The evodiamine was detected on 254nm and proteins was on 196nm、254nm by HPCE. The electrophoretic parameters were optimized by studying the efffect of the buffer composition. Results: Calibration graph was constructed in the range 1.944 μg·mL -1～7.766 μg·mL -1 for evodiamine (r=0.9974). The regression equation was y= 188.7x-32.1. The average recovery was 101.3 (n=3). In this paper, the proteins of Frutus Evodia HPCE-FPS was established at 196nm and 254nm. Conclusion: Content of evodiamine from Fructus Evodia was determined by HPCE-CZE and HPCE-FPS and distinguished three species from different growing areas related to intrinsic quality of them. The research provides new technique for evaluating quality of traditional Chinese medicine.

关 键 词：吴茱萸 吴茱萸碱 蛋白类物质 HPCE—CZE HPCE—FPS 

分 类 号：R284.1[医药卫生—中药学]