水通道蛋白1在大鼠肺泡Ⅱ型细胞上的表达  

Expression of aquaporin-1 in rat alveolar typeⅡ cells

作　　者：李涛平[1] 刘琳[1] 

机构地区：[1]中国人民解放军第一军医大学南方医院呼吸科,广东广州510515

出　　处：《中国急救医学》2003年第8期522-524,共3页Chinese Journal of Critical Care Medicine

基　　金：国家自然基金课题 (No 3 9971789)

摘　　要：目的　观察大鼠肺泡Ⅱ型上皮细胞水通道蛋白 1(AQP1)的表达。方法　对肺泡Ⅱ型上皮细胞进行分离、纯化 ,通过免疫组化及免疫电镜方法检测AQP1在该细胞上的表达。结果　大鼠肺泡Ⅱ型细胞免疫组化AQP1呈阳性 ,免疫电镜细胞膜上可见高电子密度阳性反应。结论　AQP1存在于大鼠肺泡Ⅱ型细胞膜上 ,对呼吸系统水转运可能起到重要作用。Objective To explore the expression of aquaporin-1(AQP1) in rat alveolar type Ⅱ cells. Methods Rat alveolar type Ⅱ cells were isolated and purified, and the distribution of AQP1 in alveolar type Ⅱ cells were observed by immunocytochemistry and immunoelectron microscope with affinity-purified antibodies to human AQP1. Results Immunocytochemistry showed that AQP1 was stained in alveolar type Ⅱ cells, and immunoelectron microscope demonstrated that AQP1 was present at the microvilli and apical membrane of type Ⅱ cells. Conclusion AQP1 existed in the alveolar type Ⅱ cells of the rats, it might play a role in pneuma fluid transportation.

关 键 词：水通道蛋白 肺泡Ⅱ型细胞 

分 类 号：R56[医药卫生—呼吸系统]