中国北京人群HLA-DRB1基因多态性的研究  

Study on the genic polymorphism of HLA-DRB1 in a population in the north of China

作　　者：李丹[1] 刘海燕[1] 郗斌[1] 于燕[1] 

机构地区：[1]北京大学人民医院血液病研究所HLA实验室,100044

出　　处：《中华医学遗传学杂志》2003年第4期325-327,共3页Chinese Journal of Medical Genetics

摘　　要：目的　调查北京人群人类白细胞抗原 (human leukocyte antigen,HL A) - DRB1基因多态性 ,获得完整准确的遗传学数据。方法　应用聚合酶链反应 -序列特异性引物方法对 2 810名北京出生的汉族人进行 HL A- DRB1基因型检测 ,并对随机抽取的 2 17名个体进行 DRB1高分辨分型。结果　检出 DRB1基因型 (低分辨率 ) 14个 ,DRB1等位基因亚型 (高分辨率 ) 39个 ,其中 DRB1* 0 90 12、* 15 0 1、* 0 70 11、* 0 80 32等位基因频率较高。结论　从基因水平分析了 H L A- DRB1基因的群体分布特征 ,提供了一套比较完整准确的 DRB1基因频率 ,为人类学及疾病相关性等研究提供了重要的参考数据。Objective: To investigate at the DNA level the polymorphism of HLA-DRB1 gene in Han population in Beijing of China. Methods: This study was conducted in the Hans in Beijing. A total of 2810 normal and unrelated neonates were analyzed and subjected to HLA-DRB1 low resolution typing, and from among them 217 neonates were randomly selected for DRB1 high resolution typing by polymerase chain reaction-sequence specific primers technique. Resutls: Fourteen HLA-DRB1 specificity and 39 alleles were found with HLA-DRB1 * 09012, * 1501, * 07011, * 08032 as the most common genes. Their gene frequencies and allelic distributions were compared to those observed in other ethnic populations. Conclusion: The results showed the characteristic of HLA-DRB1 distribution, and provided more comprehensive and accurate gene data that may serve as normal reference values for the population in Beijing, China.

关 键 词：HLA-DRB1 基因多态性 中国 北京 聚合酶链反应-序列特异性引物 异基因移植免疫反应 基因频率 

分 类 号：R392[医药卫生—免疫学]