钙粘附蛋白在人类早期胚胎的表达  

Expression of E-cadherin in human embryo

作　　者：刘芸[1] 邢福祺[2] 

机构地区：[1]南京军区福州总医院妇产科,福州350025 [2]第一军医大学南方医院妇产科

出　　处：《中华医学遗传学杂志》2003年第4期348-349,共2页Chinese Journal of Medical Genetics

摘　　要：目的　检测钙粘附蛋白在人类早期胚胎的表达 ,探讨钙粘附蛋白在胚胎着床及发育中的作用。方法　应用特异性单克隆抗体 ,采用间接免疫荧光方法 ,检测人种植前胚胎 (分别为 2、4、6、7、8细胞胚及囊胚 )上皮型钙粘附蛋白的表达 ,并应用激光共聚焦显微镜对荧光进行定量检测。结果　各期人种植前胚胎表面均见上皮型钙粘附蛋白表达 ,囊胚期表达明显增高。结论　人类早期胚胎在发育过程中能表达上皮型钙粘附蛋白 ,且表达量逐渐增加 ,可能对胚胎成功着床有重要意义。Objective: To investigate the role of calcium dependent adhesion molecules (cadherin) during the implantation and development of human embryo by studying the expression of E-cadherin in human embryo and blastocysts. Methods: Expression of E-cadherin in preimplantation embryos and blastocysts was detected by indirect immunofluorescence assay and quantitated by laser scanning confocal microscopy. Results: Expression of E-cadherin was found in all the preimplantation embryos and blastocysts the present authors studied. The expression was higher in blastocysts than that in preimplantation embryos. Conclusion: The above results suggested that human embryos and blastocysts could express E-cadherin and the expression increased during their development. These may be of significance to the adhesion and implantation of the human embryo and blastocysts.

关 键 词：钙粘附蛋白 人胚胎 着床 发育 间接免疫荧光 激光共聚焦显微镜 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]