哮喘病人的HLA-DRB、LMP、TNFβ基因遗传多态性  

HLA-DRB、LMP、TNFβ Alleles Polymorphism in Susceptibility to Asthma in Guangdong Chinese

作　　者：徐霞[1] 陈盛强[1] 刘利东[1] 孙宝清[2] 陈顺存[2] 

机构地区：[1]广州医学院检验系,广州510182 [2]广州市呼吸疾病研究所,广州510182

出　　处：《现代临床医学生物工程学杂志》2003年第3期188-190,共3页Journal of Modern Clinical Medical Bioengineering

摘　　要：目的　探讨人类HLA -DRB、LMP、TNFβ基因型在广州地区支气管哮喘患者中的分布 .方法　采用序列特异性引物聚合酶链反应 (PCR -SSP)和PCR -RFLP技术检测HLA -DRB和LMP、TNFβ的等位基因在 5 2例哮喘患者和10 3例健康者间的分布 .结果　哮喘病人HLA -DRB频率 :DR10 .0 0 96、DR2 0 .192、DR30 .0 5 76、DR4 0 .0 86 5、DR110 .0 2 88、DR130 .0 0 96、DR70 .0 4 81、DR80 .0 76 9、DR90 .115、DR10 0 .0 0 96、DR空 0 .0 5 76 .哮喘病人TNFβ基因频率为TNFβ1 0 .4 32 6 ,TNFβ2 0 .5 6 73,支气管哮喘病人LMP基因频率为 :LMP2 A 0 .345 5、LMP2 B 0 .6 5 4 5、LMP7 A0 .5 185、LMP7 B 0 .4 815 .结论　广州地区哮喘病人与HLA -DRB、LMP。Objectives To investigate whether susceptibility or resistance to asthma associated with HLA-DRB、LMP、TNFβ alleles and analyze the relationship between HLA-DRB、LMP、TNFβ genes and asthma. Methods Using PCR-SSP(sequencespecific primer polymerase chain reaction) and PCR-RFLP,we tested the frequency distribution of HLA-DRB、LMP、TNFβ alleles in 52 asthmatic patients and 103 healthy volunteers from Guangdong China. Results In asthmatic patients,It was shown that the gene frequency were as followed;HLA-DRB genefrequenc:DR1 0.0096 、DR2 0.192、DR3 0.0576、DR4 0.0865、DR11 0.0288、DR13 0.0096 、DR7 0.0481、DR8 0.0769 、DR9 0.115、DR10 0.0096、 DR 空0.0576;TNFβ gene frequency:TNFβ1 0.4326,TNFβ2 0.5673 ;LMP Genefrquenc:LMP2A 0.3455、LMP2B 0.6545、LMP7A 0.5185. There was no significantly increased gene frequency of alleles HLA-DRB、LMP、TNFβ in asthmatics compared with normal controls. Conclusions There was no relationship between HLA-DRB、LMP、TNFβ alleles and asthma in Guangdong Chinese.

关 键 词：哮喘 HLA-DRB LMP TNFΒ 基因 遗传多态性 

分 类 号：R562.25[医药卫生—呼吸系统]