联苯胺对鲫鱼肝脏线粒体影响的微量热研究  

Microcalorimetry Study on the Effect of Benzidine on Mitochondria Isolated from Liver of Carassius auratus

作　　者：戴捷[1] 周培疆[1] 朱军成[2] 刘鹏[2] 刘义[2] 

机构地区：[1]武汉大学资源与环境科学学院 [2]武汉大学化学与分子科学学院,湖北武汉430072

出　　处：《武汉大学学报（理学版）》2003年第4期437-440,共4页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目 (2 0 1770 18)

摘　　要：用TAMair等温微量热检测系统 ,测定鲫鱼 (Carassiusauratus)肝脏线粒体体外代谢热及不同浓度联苯胺 (1.0 ,5 .0 ,10 .0 ,2 0 .0 ,4 0 .0mg/L)对其代谢活性的影响 .结果表明 ,线粒体体外代谢活性恢复期速率常数k1,活性衰竭期速率常数k2 以及最大放热功率Pmax均受到联苯胺的影响 ,且随着联苯胺浓度的升高 。The metabolic thermogenic curves of the mitochondria isolated from liver of Carassius auratus and the effects of different concentration benzidine (1.0, 5.0, 10.0, 20.0, 40.0 g/mL) on the mitochondria were determined by using a TAM air microcalorimeter. The results show that benzidine brings out relevant changes of the values of the rate constant (k 1) in the activity recovery phase, the rate constant (k 2) in the activity declining phase and the maximum heat production (P max). The total heat production (Q) of every adminstrated group declines gradually while the concentration of benzidine increases.

关 键 词：联苯胺 鲫鱼 肝脏 线粒体 微量热 代谢热 代谢活性 环境化学污染物 毒性作用 

分 类 号：X520.322.5[环境科学与工程—环境工程]