大鼠胰腺β肿瘤细胞中胰岛素样生长因子结合蛋白-2和3的表达调控  

Expression and Regulation of IGFBP-2 and-3 in Insulin Producing Cell Line of Rats

作　　者：张春举[1] 程志祥[2] 熊俊[2] 倪雪峰[2] 周锦勇[2] 钟燕[2] 德伟[2] 

机构地区：[1]江苏省连云港中药学校,江苏连云港222001 [2]南京医科大学生物化学和分子生物学教研室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2003年第5期429-431,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然科学基金(BK2001119);南京医科大学创新基金(CX200005)

摘　　要：目的:研究大鼠胰腺β肿瘤细胞(INS-1)中,生长激素(GH)、催乳素(PRL)、葡萄糖和胰岛素对胰岛素样生长因子结合蛋白-2和3(IGFBP-2、3)的表达调控,探索IGFBP-2和3对维持正常β细胞生理功能的作用。方法:RNA印迹法检测INS-1细胞中IGFI3P-2和3的mRNA表达情况及蛋白免疫印迹法分析INS-1细胞中IGFBP-3和2蛋白表达。结果:在INS-1细胞中,GH和PRL调控IGFBP-3 mRNA和蛋白表达。葡萄糖对IGFBP-2 mRNA调节作用不同:高浓度(10 mmol/L)葡萄糖条件下,IGFBP-2 mRNA表达被抑制;低浓度(1.0～5.6 mmol/L)葡萄糖条件下,IGFBP-2 mRNA有表达。葡萄糖浓度为5.6 mmol/L时,加入GH孵育16 h,IGFBP-2 mRNA表达被抑制。外源胰岛素抑制 IGFBP-2 mRNA的表达。结论:GH和PRL可以调控IGFBP-3的mRNA和蛋白表达,IGFBP-2 mRNA表达主要通过胰岛素调控。Objective:To study the effects of GH, PRL, glucose and insulin on IGFBP-3 mRNA and IGFBP-2 mRNA expression in INS-1 cell. Methods: The expression of tGFBP-2,3 mRNA was detected by Northern blot and IGFBP-3 proteins was detected by method of Western blotting. Results: The expression of IGFBP-3 mRNA was up-regulated by bovine GH(bGH), rat PRL(rPRL) in the INS-1 cells. The glucose had variant effect on IGFBP-2 gene expression. On high concentration of glucose (10 mmol/L), the expression of IGFBP-2 mRNA was suppressed. On low concentration of glucose (1.0-5.6 mmol/L), expression of IGFBP-2 mRNA was normal. After incubation 16 h with exogenous GH the expression of IGFBP-2 was inhibited. The expression of IGFBP-2 mRNA was inhibited by exogenous insulin at low glucose concentration. Conclusion:The expression of IGFBP-3 mRNA is regulated by GH and PRL and the expression of IGFBP-2 mRNA is mainly regulated by insulin.

关 键 词：胰岛素样生长因子结合蛋白-2 胰岛素样生长因子结合蛋白-3 葡萄糖 胰腺Β细胞 大鼠 表达调控 

分 类 号：Q578[生物学—生物化学] Q786