GRP1.8融合反义4CL1基因调控烟草木质素生物合成  

Modulate the lignin biosynthesis by expression GRP1.8 promoter:anti-4CL1 gene in transgenic tobacco.

作　　者：赵艳玲[1] 陆海[1] 陶霞娟[1] 陈雪梅[1] 蒋湘宁[1] 

机构地区：[1]北京林业大学生物科学与技术学院

出　　处：《北京林业大学学报》2003年第4期16-20,共5页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目 ( 3 0 2 710 66);"973"国家重大基础研究项目 (G19990 160 0 5 );国家自然科学基金重点项目 ( 3 973 0 3 5 0 )共同资助

摘　　要：该研究成功地将从刺槐 (Robiniapseudoacacia)中克隆得到的定位于形成层表达的GRP1 8启动子 ,分别与GUS报告基因及从毛白杨 (Populustomentosa)中克隆的香豆酸连接酶 (4CL1)基因连接构建成GRP1 8 GUS和GRP1 8 anti 4CL1融合基因 ,转基因于烟草植物 .经组织化学检测分析 ,GUS基因成功地由GRP1 8启动子驱动定位于形成层表达 ;融合基因GRP1 8 anti 4CL1的表达明显地改变了转基因烟草植株的木质素和纤维素的含量和比例 .转基因烟草的木质素含量较对照平均降低了 13 7% ,而转基因植株的纤维素含量较对照升高了 15 6 % .coumarate coenzyme A ligase plays a key role in the lignin phenylpropanoid metabolism. Plant expression binary vector was constructed by inserting vascular specific expression promoter of the glycine rich protein (GRP1 8) from Robinia pseudoacacia fused with GUS reporter gene and antisense 4 coumarate:CoA ligase (4CL1) gene from Populus tomentosa, respectively. Regenerated tobacco plants were verified by PCR procedure, histochemically detected by GUS staining and qualitatively analysis of lignin and cellulose content by chemistry analysis procedure. Compared to the wildtype control plants, the lignin content reduced by 13 7% while cellulose increased by 15 6% in the GRP1 8 anti 4CL1 fusion transgenic tobacco.

关 键 词：形成层定位表达启动子GRP1.8 反义4CL1基因 转基因烟草 木质素 纤维素 

分 类 号：Q544[生物学—生物化学]