海藻糖的MTSase及MTHase酶促合成条件的研究  

Conditions of Biosynthesis of Trehalose by MTSase and MTHase

作　　者：赖承兴[1] 葛宇[1] 袁勤生[1] 

机构地区：[1]华东理工大学生物反应器国家重点实验室,应用生物化学研究室上海200237

出　　处：《中国医药工业杂志》2003年第9期433-435,共3页Chinese Journal of Pharmaceuticals

摘　　要：本室收藏一株产 MTSase和 MTHase两酶并能将淀粉水解物转化为海藻糖的菌株 Micrococcus luteus。实验发现 ,菌体在对数生长期细胞内的 MTSase和 MTHase累积量极少 ,但在其基本停止生长后继续培养 12 h,胞内MTSase和 MTHase两酶含量急剧增加。两酶联合作用的最适反应条件为 p H6 .5 ,温度 4 0℃ ;最适底物是 DE值为11.6、浓度为 15 %的淀粉水解液。A couple of enzymes, MTSase and MTHase, capable of converting partially hydrolyzed starch into trehalose, were obtained from a strain sample Micrococcus luteus preserved in our laboratory. The research revealed that MTSase and MTHase were scacely accumulated in the cell during the course of the cells rapid growth, however the content of the two enzymes increased quickly by culturing the cells for 12 h after the cells almost stopped growing. The optimum conditions for the biosynthesis were: pH 6.5, 40℃ and 15%( w/v) of the starch hydrolysate(DE value 11.6).

关 键 词：低聚麦芽糖苷基海藻糖合成酶(MTSase) 低聚麦芽糖苷基海藻糖水解酶(MTHase) 海藻糖 淀粉水解液 

分 类 号：Q814[生物学—生物工程] Q815