高效液相色谱法测定心肌腺苷酸含量  

HPLC determination of adenosine phosphates in mouse myocardium

作　　者：陈建斌[1] 饶邦复[1] 周远大[2] 何海霞[2] 

机构地区：[1]重庆医科大学临床学院内科,重庆400016 [2]重庆医科大学临床学院临床药理研究室,重庆400016

出　　处：《重庆医科大学学报》2003年第4期485-486,490,共3页Journal of Chongqing Medical University

摘　　要：目的 :用改良高效液相色谱法测定小鼠心肌腺苷酸含量。方法 :ODSC18柱 ,检测波长 2 5 4nm。甲醇 - 5 0mmol/L磷酸盐 (1∶99)缓冲液 (pH6 .0 7)为流动相。 结果 :心肌ATP、ADP和AMP平均回收率分别为 10 1.1%± 9.3%、94 .8%± 10 .6 %及 98.3%± 5 .7% ;日内RSD分别为 3.5 %、6 .9%和 4 .8% ;日间RSD分别为 6 .4 %、8.6 %及 8.2 %。结论 :该方法简便、快速、灵敏。采用该法发现病毒性心肌炎小鼠心肌ATP、ADP和AMP含量降低。Objective:A method for the quantitative determination of adenosine phosphates of mouse myocardium by HPLC is described.Methods:A ODS C 18 column and a mobile phase of methol-50mmol·L -1 phosphate (1∶99)buffer solution (pH 6.07),with UV detector at 254nm were used.Results:The average recovery rates of ATP,ADP and AMP were 101.7%±9.3%,94.8%±10.6% and 98.3%±5.7% respectively;the RSDs of within-day were 3.5%,6.8% and 4.8%,and the RSDs of between-days were 6.4%,8.6% and 8.2%,respectively.Conclusion:The method is simple,rapid and senstive and can be used to analyse the adenosine phosphates in myocardium.The myocardial contents of ATP,ADP as well as AMP decline notably in mice with viral myocarditis.

关 键 词：高效液相色谱法 腺苷酸 

分 类 号：R927.1[医药卫生—药学]