高效液相色谱法测定氢化可的松发酵液中的氢化可的松及其有关物质  

Determination of Hydrocortisone and Related Compounds in the Culture of Hydroc ortisone by High Performance Liquid Chromatography

作　　者：卢文玉[1] 郭亚文[1] 王敏[1] 杜连祥[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222

出　　处：《色谱》2003年第4期388-390,共3页Chinese Journal of Chromatography

基　　金：天津市科技攻关重点项目(963106811);教育部科技研究重点项目(天津01004).

摘　　要：建立了用高效液相色谱法对氢化可的松发酵液中的主要组分氢化可的松(HC)、表氢化可的松(EHC)、17α 羟基孕甾 4 烯 3,20 二酮 21 醋酸酯(RSA)和17α 羟基孕甾 4 烯 3,20 二酮(RS)进行测定的方法。采用硅胶吸附色谱柱,以二氯甲烷 乙醚 甲醇 水(体积比为385∶60∶30∶2)为流动相,流速0 8mL/min,在242nm波长下检测。发酵液各组分分离良好,HC,EHC,RSA和RS的线性范围分别为25～375mg/L,5～75mg/L,5～75mg/L和5～75mg/L;检出限分别为0 4,0 1,0 05和0 02mg/L。方法准确,灵敏,重现性好。A method for the determination of hydrocortisone (HC), epihydrocortisone (EHC), 17α-hydroxypregn-4-en-3,20-dione-21-acetate (RSA) and 17α -hydroxypregn-4-en-3,20-dione (RS) in the culture by high performance l iquid chromatography (HPLC) was developed. The HPLC was performed on a Phenomene x Prodigy Silica 100A column (5 μm, 250 mm×46 mm id) by using dichlorome thane-ether-methanol-water (385∶60∶30∶2, v/v) as mobile phase. The flow r ate was 08 mL/min and the UV detection wavelenth was 242 nm. The method ha s been successfully used for the determination of the four compounds with rec overies of 1009%, 968%, 1005% and 1006% for HC, EHC, RSA and RS, respect ively. The linear ranges of HC, EHC, RSA and RS were 25-375 mg/L, 5-75 mg/ L,5-75 mg/L and 5-75 mg/L with the detection limits of 04, 01, 0 05 and 002 mg/L, respectively. This method is accurate, sensitive and reproducible.

关 键 词：高效液相色谱法 测定 氢化可的松发酵液 氢化可的松 组分 分离 皮质激素类药物 

分 类 号：R977.11[医药卫生—药品] R927.2[医药卫生—药学]