人脐血间充质干/祖细胞诱导为心肌样细胞的实验研究  

Induced differentiation of human cord blood mesenchymal stem/progenitor cells into cardiomyocyte-like cell in vitro

作　　者：程范军[1] 邹萍[1] 杨汉东[2] 余正堂[2] 仲照东[1] 

机构地区：[1]华中科技大学同济医学院协和医院血液病研究所,武汉430022 [2]华中科技大学同济医学院东风临床学院

出　　处：《中华器官移植杂志》2003年第4期220-222,共3页Chinese Journal of Organ Transplantation

摘　　要：目的　了解脐血间充质干 /祖细胞用于心肌细胞再生的可行性 ,并探讨其最佳的诱导培养条件。方法　将脐血单个核细胞置于低血清 (2 % )DMEM培养基中生成贴壁细胞层 ,依传代方法 ,用相同培养条件进行扩增 ,扩增后的贴壁细胞置入心肌诱导培养液中 ,并添加 5 氮杂胞苷进行诱导分化、采用心肌特异性收缩蛋白 肌钙蛋白T染色鉴定被诱生的心肌样细胞。结果　脐血间充质干 /祖细胞克隆在脐血单个核细胞中出现频率为 0 .5× 10 -6,在传 2 0代时 ,可有效扩增 1.3× 10 7倍 ,诱导后 ,70 %的脐血间充质干 /祖细胞分化为心肌样细胞。结论　采用上述扩增与诱导条件 ,脐血间充质干 /祖细胞可得到有效扩增 ,并可高效向心肌样细胞分化。Objective To investigate the feasibility of using CB-MSPCs to regenerate cardiomyocyte and to define their optimal inducing condition. Methods The CB mononuclear cell was cultured in low serum (2%) DMEM medium to produce an adherent layer. These adherent cells were expanded and put in cardiomyocyte inducing medium supplemented with 5-azacytidine. Cardiogenic specific contractile protein troponin T staining was used to identify cardiomyocyte-like cells. Results MSPCs were presented in CB mononuclear cells at frequency of 0.5 ×10 -6 , and could be extensively expanded about 1.3 ×10 7 times within 20 sub-cultivation. After cardiogenic induction, 70% CB-MSPCs were differentiated into cardiomyocyte-like cells. Conclusion Low serum culture could expand CB-MSPCs extensively and the expanded CB-MSPCs could be induced to be differentiated into cardiomyocyte-like cells in high efficiency by using the inducing condition in this study.

关 键 词：人脐血间充质干/祖细胞 心肌细胞再生 诱导培养条件 细胞分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]