重楼对宫颈癌细胞钙信号的影响  

Influence of Rhizoma Paridis on Calcium Messenger System in Hela Cells

作　　者：高冬[1] 高永琳[2] 白平[1] 

机构地区：[1]福建中医学院现代医学部,福建福州350003 [2]福建医科大学生化与分子生物学系,福建福州350004

出　　处：《福建中医学院学报》2003年第4期26-28,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：福建省卫生厅青年基金资助项目 ( 3 2 0 2 1980 2 1)

摘　　要：为探讨重楼对宫颈癌 (Hela)细胞钙信号的影响及其抗癌作用机理。利用荧光染料Fura 2在细胞内能与游离钙结合 ,在一定波长光的激发下可发出荧光的特性 ,根据荧光的强度来检测游离钙浓度的变化 。To study the influence of rhizoma paridis(RP)on calcium messenger system in Hela cells and its mechanism of anticancer,the characteristics that fluorochrome Fura 2 could combine with free Ca\+\{2+\} and give out of fluorescence under some wavelength light stimulation was used,and then the changes of free Ca\+\{2+\} were determined according to intensity of fluorescence.The results showed that RP could stimulate the in flow of extracellular Ca\+\{2+\},and then improve the cytoplasmatic free Ca\+\{2+\} density.

关 键 词：重楼 宫颈癌 游离钙 浓度检测 瘤细胞 细胞增殖 植物药 

分 类 号：R273[医药卫生—中西医结合]