头孢他啶对早期和成熟期绿脓假单胞菌生物膜菌作用的差别  

Different effect of Ceftazidime on the early and mature Pseudomonas aeruginosa biofilm bacteria

作　　者：张海平[1] 胡必杰[1] 李华茵[1] 陈雪华[1] 周昭彦[1] 董荔[1] 何礼贤[1] 

机构地区：[1]复旦大学附属中山医院呼吸科

出　　处：《中华检验医学杂志》2003年第6期349-351,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的　观察头孢他啶对早期绿脓假单胞菌 (PA)生物膜 (BF)细菌和成熟期PABF细菌作用的差别。方法　通过恒化器结合改良的Robbins装置分别建立早期和成熟期PABF ,用 64μg/ml头孢他啶分别作用于两种生物膜细菌 2 4h ,扫描电镜观察结果 ,并分析生物膜内活菌数的变化。结果　头孢他啶作用 2 4h后 ,扫描电镜观察显示早期PABF内的细菌已基本被清除 ,活菌清除率为99 62 % ,而成熟期PABF内仍有大量细菌存在 ,其活菌清除率仅为 10 0 2 % ,两者差异有显著性。结论　成熟期PABF细菌比早期PABF细菌对头孢他啶具有更强的抵抗性。Objective To observe the effect of Ceftazidime on the early and mature Pseudomonas aeruginosa biofilm bacteria. Method The early and mature PA biofilm were formed through the chemostat combined Robbins device. Then 64 μg/ml Ceftazidime was applied to the early and mature PA biofilm bacteria for 24 hours respectively. Scanning electronic microscope(SEM) was used to observe the effect of Ceftazidime on early and mature biofilm. The difference between the viable bacteria in early and mature biofilm was analyzed by statistical method. Result 24 hours after Ceftazidime was added, Pseudomonas aeruginosa in early biofilm was killed on the whole while there were still numbers of bacteria in mature biofilm. Statistical analysis demonstrates a great variance in viable counts. Conclusion Pseudomonas aeruginosa in mature biofilm is more tolerant to antibiotics than that in early biofilm.

关 键 词：头孢他啶 早期绿脓假单胞菌 生物膜 PABF细菌 医院感染 

分 类 号：R96[医药卫生—药理学]