ZFX、ZFY基因与动物进化关系的分析  

Analyzed Sequence of ZFY and ZFX Gene Homologous in Several Species Comparatively

作　　者：徐亚欧[1] 王永[1] 郑玉才[1] 熊永[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《四川畜牧兽医》2003年第8期16-17,19,共3页Sichuan Animal & Veterinary Sciences

基　　金：国家自然科学基金项目<放牧条件下牦牛乳生理生化特点研究>(编号39870607)

摘　　要：本文对ChinaHolsteinZFY,ZFX基因外显子片段进行克隆、测序,并利用NCBI和BLAST犤5、6、7犦与Japaneseblack、Sheep、Horse、Pig、Human的ZFY、ZFX的同一外显子同源序列进行对比分析,结果表明:本文分析的锌指蛋白基因ZFY和ZFX外显子片段在上述各物种间,种属距离越远,种属间SNP的差异越大。对ChinaHolstein及Japaneseblack间的ZFY、ZFX对比分析表明:同种属内不同品种间的SNP也存在差异,但同源性高于种间。同种属或品种内ZFY与ZFX的同源比较ZFY的SNP高于ZFX,ZFY在进化过程中较ZFX更活跃,而ZFX较为保守,该结果与国外多数学者的研究结果一致犤2、3、13犦,对ZFY与ZFX的SNP性质进行研究发现ZFY中的碱基颠换率为16.22%、ZFX为16%.本文结果可作为奶牛胚胎早期性别鉴定的基础方法。We have sequenced the partial exon of the zinc finger genes(ZFY and ZFX)in China Holstein and obtained another in Japanese-black、Sheep、Horse、Pig and Human from NCBI.Among these species,the percentage of the nucleotide sequence similarities of the ZFX gene were93.13～99.61%and90.19～97.65%for the ZFY gene.Of 255sites in the ZFX and ZFY gene sequences,25for ZFX gene and37for ZFY gene were found to be variable.The racial distance is longer the variance is bigger.The rates of synonymous substitution per site were higher in the ZFY exon than in the ZFX exon in these species.Comparison of the ZFX and ZFY genes revealed that the synonymous substitution levels were different between China Holstein and Japanese-black of bos tarrus.

关 键 词：ZFX基因 ZFY基因 动物进化 种属距离 对比分析 碱基颠换率 奶牛 胚胎 早期性别鉴定 

分 类 号：Q953[生物学—动物学]