四物汤及两味中药组方对γ射线照射所致血虚证小鼠作用机制的研究  

Mechanism research of Siwu Decoction and every combination of two herbs in it on mice with blood deficiency syndrome induced by γ-ray

作　　者：朱力军[1] 路晓钦[1] 谭洪玲[1] 肖成荣[1] 高月[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《天津中医药》2003年第4期58-61,共4页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目 (30070913)

摘　　要：[目的]探讨四物汤及其两味药组方治疗血虚证小鼠的补血作用机制。[方法]采用 60Coγ射线全身照射制成实验性小鼠血虚证模型 ,测定外周血象、CD34+ 细胞、细胞周期、NFS60细胞增殖以及造血干祖细胞的增殖情况。[结果]1)四物汤在各指标上都优于其两味药组方 ;(2)两味药组方中 ,当归相关组对CFU -E ,CFU -Mix ,CFU -GM的促进作用较好 ,且有升高白细胞的作用 ;而地黄相关组对CFU -E有增殖作用 ;白芍相关组能明显促进NFS60细胞的增殖 ;川芎相关组增加CD34抗原表达 ,并促进细胞由G0/1 期向S期转化 ,其作用和所含的川芎嗪有关。[结论]四物汤全方优于两味药组方 。Objective To study the mechanism of Siwu Decoction and every combination of two herbs in it on mice with blood deficiency syndrome. The blood deficiency model was by systemic irradiation of 60Coγ-ray on mice. Peripheral hemogram, CD34+ cell, cell cycle, proliferation of NFS60 cell and hemopioetic cell were observed. 1) All indexes of Siwu Decoction was superior to that of other combination. 2) The combination related with Danggui could improve CFU-E, CFU-Mix and CFU-GM and enhance WBC. The combination related with Dihuang could promote the proliferation of CFU-E. The combination related with Baishao could improve the proliferation of NF60 cell markedly. The combination related with Chuanxiong could promote CD34 antigen expression and accelerate cell transformation from G0/1phase to S phase. The effect of the last combination was associated with ligustrazine. [Conclusion] The whole decoction, Siwu Decoction is superior to other combination. The effect target and the effect strength of each combination is different.

关 键 词：四物汤 两味中药组方 Γ射线照射 血虚证 小鼠 动物模型 

分 类 号：R255.7[医药卫生—中医内科学] R285.5[医药卫生—中医学]