水稻光温敏核不育系的ISSR和SSR遗传分析比较  

Comparisons of Genetic Analysis Based on ISSR and SSR in PGMS and TGMS Rice Lines

作　　者：李进波[1,2] 江良荣[2] 李春海[3] 牟同敏[3] 

机构地区：[1]湖北省农科院杂交水稻工程技术研究中心 [2]海南省农作物分子育种重点实验室,三亚572025 [3]华中农业大学作物遗传改良国家重点实验室

出　　处：《分子植物育种》2003年第1期42-47,共6页Molecular Plant Breeding

基　　金：国家863计划(批准号:2001AA211091);海南省自然科学基金项目(批准号:3003);2001年海南省科学事业费项目资助。

摘　　要：本研究应用ISSR和SSR技术建立了 2 4个水稻光温敏核不育系的DNA指纹图谱 ,利用 13个ISSR引物和 2 0对SSR引物 ,分别获得 174个多态性片段和 6 2个多态性片段 ,平均每个ISSR引物检测到13 38个多态性片段 ,远远高于SSR引物的检测率。根据遗传距离进行的聚类分析表明 ,利用这两种标记所得的聚类结果十分相似 ,2 4个材料被聚为粳型 ,偏籼型和籼型三个类群 ,在籼型不育系类群内 ,又可明显的分成三个亚类群 ,其中 7个安农S - 1衍生的不育系聚为一类 ,与农垦 5 8S衍生的不育系有明显的遗传差异。根据两种标记计算的遗传距离及其遗传关系 ,所得的结果仍有一些差异 ,但总体趋势是一致。研究结果表明 ,ISSR和SSR标记适用于构建DNA指纹图谱 ,进行分类鉴定和遗传分析。In this study, DNA fingerprints of 24 elite photoperiod sensitive genic male sterile (PGMS) and thermo sensitive genic male sterile (TGMS ) rice lines were established using inter simple sequence repeats (ISSR) markers and simple sequence repeats (SSR) markers. 13 ISSR primers could amplify 174 polymorphic bands and 20 SSR primers could amplify 62 polymorphic bands. The numbers of average polymorphic bands produced by per ISSR primer are 13 38 more than those produced by per pair of SSR primer. The results showed that clustering analysis based on ISSR data is very similar to that based on SSR data. These 24 sterile lines used in this research were divided into three groups, indica group, indica like and japonica group. In the indicagroup, 19 sterile lines were also clustered into three subgroups. 7 sterile lines derived from Annong S 1 were clustered together, which show obviously genetic differences compared to 11 indica sterile lines derived from Nongken 58S. There are a little differences in genetic distance and genetic relationship of tested sterile lines calculated by depending on data from ISSR or SSR. The results of this research demonstrated that it is more suitable for using ISSR and SSR as marker to do DNA fingerprint construction, classification and genetic analysis.

关 键 词：水稻 光温敏核不育系 ISSR SSR 遗传分析 遗传标记 DNA指纹图谱 

分 类 号：S511[农业科学—作物学]