杀伤细胞免疫球蛋白样受体基因多态性与血清阴性脊柱关节病的关联性研究  

Association of killer cell immunoglobulin-like receptor gene polymorphisms with seronegative spondylarthropathies

作　　者：龚卫娟[1] 杨珏琴[1] 张磊[1] 姚芳娟[1] 范丽安[1] 

机构地区：[1]上海第二医科大学上海市免疫学研究所免疫遗传室,200025

出　　处：《中华风湿病学杂志》2003年第8期465-468,共4页Chinese Journal of Rheumatology

基　　金：国家自然科学基金资助项目 ( 3 9970 742 ) ;上海市高等学校科技基金资助项目 ( 99QB68) ;上海 联合利华研究与发展基金资助项目 ( 9911)

摘　　要：目的　探讨杀伤细胞免疫球蛋白样受体 (KIR)基因多态性与血清阴性脊柱关节病(SpA)的发生是否存在关联。 方法　采用聚合酶链反应 (PCR) /系列特异性引物 (SSP)方法 ,调查上海地区 197例HLA B2 7(+)SpA患者和 87名种族匹配的正常对照KIR基因位点的多态性。结果　SpA病例组中KIR3DL1基因的频率 (0 76 3)比对照组 (1 0 0 )显著降低 (RR =0 76 ,P =0 0 0 3) ,两个假基因KIR2DP1和KIR3DP1的频率较对照组明显升高 (RR =1 1,P =0 0 0 4 ) ,而其他各KIR基因频率差异无显著性。Objective To investigate whether the killer cell immunoglobulin like receptor (KIR) gene polymorphisms are associated with seronegative spondylarthropathies.Methods All 197 HLA B27 positive patients with seronegative spondyloarthro pathies (SpA) and 83 randomly ethnically matched healthy controls were enrolled to detect the KIR genotype using PCR SSP in Shanghai area.Results The KIR3DL1 gene frequency was significantly lower in the patient group (0 763) than in the control group (1 00) (RR=0 76, P =0 003).Meanwhile,the gene frequencies of two pseudogenes (KIR2DP1,KIR3DP1) were significantly higher in SpA group than in healthy control group (RR=1 1, P =0 004).Conclusion There may be a negative association between pathogenesis of SpA and KIR3DL1 gene.The function of KIR3DL1 molecule should be investigated deeply.

关 键 词：杀伤细胞 免疫球蛋白样受体 基因多态性 血清阴性脊柱关节病 

分 类 号：R684[医药卫生—骨科学]