正、反杂交二西蜂及其父、母本DNA基因组的多态性研究  

Study on DNA Polymorphisms of Cis and Trans Hybridization and their Parents

作　　者：戴英[1] 陆挺[1] 张高川[1] 蒋滢[1] 葛风晨[2] 薛运波[2] 

机构地区：[1]苏州大学生命科学院,江苏苏州215007 [2]吉林省养蜂科学研究所,吉林吉林132108

出　　处：《生物技术》2003年第4期10-11,共2页Biotechnology

基　　金：江苏省自然科学基金资助项目 (BK2 0 0 0 0 2 6)

摘　　要：使用RAPD -PCR (randomamplifiedpolymorphicDNA—polymerasechainreaction)技术 ,分析了正杂交松丹一号西蜂 ,反杂交松丹二号西蜂及其父、母本的DNA多态性 ,结果正杂交松丹一号西蜂 ,反杂交松丹二号西蜂及其父、母本的DNA多态性均有明显的差别 ,这说明通过正杂交松丹一号与反杂交松丹二号的DNA分子发生了变异。This paper adopted RAPD-PCR techniques and analysed DNA Polymorphisms of cis hybridization Song Dan 1 honey bees,trans hybridization Song Dan 2 honey bees and their parents.The results showed that DNA polymorphisms of Song Dan 11differentiated from Song Dan 2 and their parents.It is proved that DNA molecule of Song Dan 1 and 2 had obvious mutation arousing by hybridization.

关 键 词：西蜂 DNA多态性 正杂交松丹一号 反杂交松丹二号 

分 类 号：S891[农业科学—特种经济动物饲养]