石蜡包埋人体组织DNA分型的研究  

Research on genotyping of DNA extracted from FFPE

作　　者：周毅[1] 严红[1] 李万水[1] 张大安[1] 李俊涛[1] 

机构地区：[1]公安部物证鉴定中心,北京100038

出　　处：《刑事技术》2003年第4期17-18,共2页Forensic Science and Technology

摘　　要：目的研究石蜡包埋人体组织的DNA提取方法对其STR分型的影响,并建立石蜡包埋组织的DNA提取方法。方法经不同预处理条件后采用Chelex-100法提取石蜡包埋人体组织中的DNA,用不同的反应体系进行STR复合扩增检验。结果经福尔马林固定、石蜡包埋的人体组织直接采用Celex-100法提取DNA与65℃水浴除蜡后采用Celex-100法提取DNA均可获得满意的DNA分型。结论该方法简便易行,实验效果好,适合检案。Objective To research different DNA extraction methods from formalin fixed paraffin embedded(FFPE)tissues and their effects on genotyping.Method DNA was extracted with Chelex-100protocol after various pretreatments and PCR was done in different volumes.Results Both the Chelex-100extraction procedure with the FFPE tissues incubated at65℃(deparaffinized)and the direct Chelex-100extraction procedure could result in reliable STR typing.Conclusion This method is easy to operate and useful for casework.

关 键 词：DNA提取 石蜡包埋组织 STR分型 影响因素 复合扩增检验 预处理条件 

分 类 号：D919.2[医药卫生—法医学]