两种虎纹捕鸟蛛昆虫毒素的分离纯化及生物学活性鉴定  

Purification and Characterization of Huwentoxin-Ⅶ and Huwentoxin-Ⅷ,Two Novel Insecticidal Neurotoxins from the Chinese Bird Spider Selenocosmia huwena

作　　者：戴捷[1] 梁宋平[1] 

机构地区：[1]湖南师范大学生命科学学院,长沙410081

出　　处：《中国生物化学与分子生物学报》2003年第4期488-492,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然基金资助项目 (No .3 93 70 15 2 )~~

摘　　要：结合离子交换和反相高效液相色谱从虎纹捕鸟蛛粗毒分离纯化到 2种虎纹捕鸟蛛毒素 ,命名为虎纹捕鸟蛛毒素 Ⅶ和虎纹捕鸟蛛毒素 Ⅷ .经质谱测定这 2种毒素的分子量分别为 3981 0 2和 4 171 12 .氨基酸序列分析发现 ,这 2种虎纹捕鸟蛛毒素同源性非常高 ,只有 6个残基位点的不同 .虎纹捕鸟蛛毒素 Ⅶ和虎纹捕鸟蛛毒素 Ⅷ的生物学功能相似 ,都能对蝗虫起到麻痹作用 ;对小鼠的中枢神经作用高剂量能使小鼠产生致死 ,但低剂量虎纹捕鸟蛛毒素 Ⅷ能使小鼠产生惊厥反应 ,而低剂量虎纹捕鸟蛛毒素 Ⅶ不能使小鼠产生惊厥反应 ;这 2种毒素都能阻断小鼠离体膈神经膈肌的神经肌肉传递 ,且与虎纹捕鸟蛛毒素Huwentoxin Ⅶ and Huwentoxin Ⅷ were isolated from the crude venom of the spider Selenocosmia huwena by the combination of ion exchange and reverse phase HPLC. The masses of the two toxins are 3 981 02 and 4 171 12 by MALDTI TOF MS. The sequences of the two toxins are very similar except that six residues are different. The biological functions of the two toxins are similar too. They paralyze locust, cause death in mouse central nervous system (CNS) in high dosage, but in low dosage huwentoxin Ⅷ can cause convulsion reaction, while huwentoxin Ⅶ cannot. Both of them can block neuromuscular transmission in an isolated mouse phrenic nerve diaphragm preparation, and also react cooperatively to the activity of huwentoxin I.

关 键 词：虎纹捕鸟蛛昆虫毒素 分离 纯化 生物学活性 氨基酸序列 

分 类 号：Q966[生物学—昆虫学] R996.3[医药卫生—毒理学]