血红素对人脐静脉内皮细胞氧化应激损伤的保护作用  

Protection of Hemin Against Oxidative-stress Damage in Human Umbilicus Vein Endothelial Cells

作　　者：周宏博[1] 王秀宏[1] 王爱民[1] 刘明[1] 申峰[1] 吕昌莲[1] 吕丹[1] 周虹[2] 

机构地区：[1]哈尔滨医科大学生物化学与分子生物学教研室,哈尔滨150086 [2]军事医学科学院野战输血研究所,北京100850

出　　处：《中国生物化学与分子生物学报》2003年第4期528-532,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：黑龙江省自然科学基金资助项目 (No.0 1 2 2 );全国高校骨干教师资助计划 2 0 0 0~~

摘　　要：采用过氧化氢作用于人脐静脉内皮细胞 ,成功地制作了氧化应激损伤的细胞模型 ,将培养的细胞分为 3组 :正常对照组 ,损伤组 (给予过氧化氢 )和血红素组 (同损伤组的制备 ,预先加入血红素 ) .观察各组细胞在光、电镜下形态学和蛋白质电泳的改变 ,用MTT法检测了各组细胞的生长状态 .结果表明 :在血红素组 ,细胞生长状态、MTT水平和蛋白质电泳结果与损伤组相比有明显好转且差异显著 ,证明血红素对人脐静脉内皮细胞氧化应激损伤有明显的保护作用 .The cell model of oxidative\|stress damage was made successfully by hydrogen peroxide in human umbilicus vein endothelial cells.The cultured cells were divided into three groups:control group,damage group in whicah H\-2O\-2 was given,and hemin group in which hemin was added previously.The cell growth conditions in the three groups were observed under the light microscope and the electron microscope.The cross\|linking proteins were analyzed by SDS\|PAGE and the surviving cells counted by MTT.There were significant differences between damage group and hemin group in cell growth condition,MTT and SDS\|PAGE pattern.The results demonstrated that hemin could protect the human umbilicus vein endothelial cells against oxidative\|stress damage.

关 键 词：血红素 人脐静脉内皮细胞 氧化应激损伤 保护作用 抗乳化保护 

分 类 号：Q592.1[生物学—生物化学] Q26