蛋白激酶B在小鼠1-细胞期受精卵中活性及表达变化  

Activity and Expression Changes of Protein Kinase B in 1-cell Stage Fertilized Eggs of Mouse

作　　者：陈菲[1] 于爱鸣[1] 冯晨[1] 付伟[1] 赵咏梅[1] 袁莹[1] 武迪迪[1] 于秉治[1] 

机构地区：[1]中国医科大学基础医学院生物化学教研室,沈阳110001

出　　处：《中国生物化学与分子生物学报》2003年第4期542-545,共4页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家重点基础研究发展规划 (973 )项目(No .G19990 5 5 90 0 2 )资助~~

摘　　要：The role of protein kinase B (PKB) in the regulation of early development of fertilized eggs in mouse and its biological function on the development of mouse 1\|cell stage fertilized eggs were studied.The kinase activity of PKB was detected,and mRNA and protein level of PKB both in MⅡ oocytes and 1\|cell stage fertilized eggs were semi\|quantitatively assayed by Western blotting and RT\|PCR.10 μmol/L LY294002,the inhibitor of PKB,was microinjected into 1\|cell stage fertilized eggs of mouse to observe its effect on the development of mouse 1\|cell stage fertilized eggs.The results showed that the activity of PKB increased obviously after the fertilization,the mRNA and protein level of PKB also increased obviously in 1\|cell stage fertilized eggs,while in MⅡ oocytes the mRNA level was not detected.The protein level was lower than that in the 1\|cell stage fertilized eggs.After microinjecting LY294002 the development from 1\|cell stage to 2\|cell stage was inhibited obviously.All the results demonstrate that PKB exists in the fertilized eggs,and the activity,expression and transcription level increase after fertilization.PKB may play a positive role during the early development of fertilized eggs in mouse from 1\|cell to 2\|cell stage,but the mechanism is still unknown.These experiments are helpful for the further study of PKB on the regulation of early development of fertilized eggs.The role of protein kinase B (PKB) in the regulation of early development of fertilized eggs in mouse and its biological function on the development of mouse 1\|cell stage fertilized eggs were studied.The kinase activity of PKB was detected,and mRNA and protein level of PKB both in MⅡ oocytes and 1\|cell stage fertilized eggs were semi\|quantitatively assayed by Western blotting and RT\|PCR.10 μmol/L LY294002,the inhibitor of PKB,was microinjected into 1\|cell stage fertilized eggs of mouse to observe its effect on the development of mouse 1\|cell stage fertilized eggs.The results showed that the activity of PKB increased obviously after the fertilization,the mRNA and protein level of PKB also increased obviously in 1\|cell stage fertilized eggs,while in MⅡ oocytes the mRNA level was not detected.The protein level was lower than that in the 1\|cell stage fertilized eggs.After microinjecting LY294002 the development from 1\|cell stage to 2\|cell stage was inhibited obviously.All the results demonstrate that PKB exists in the fertilized eggs,and the activity,expression and transcription level increase after fertilization.PKB may play a positive role during the early development of fertilized eggs in mouse from 1\|cell to 2\|cell stage,but the mechanism is still unknown.These experiments are helpful for the further study of PKB on the regulation of early development of fertilized eggs.

关 键 词：蛋白激酶B 小鼠细胞 受精卵 活性 表达 

分 类 号：Q492.6[生物学—生理学] Q253