HPLC测定槐角中槐苷含量  

HPLC Determination of Sophoricoside in Sophora japonica

作　　者：肖怀[1] 陈泽乃[2] 陆阳[2] 顾雅芳[2] 谢一凡[2] 王卓[1] 

机构地区：[1]大理学院药学院,云南大理671000 [2]上海第二医科大学化学教研室,上海200025

出　　处：《大理学院学报（综合版）》2003年第3期11-13,共3页Journal of Dali University

摘　　要：目的 :测定槐角中主要异黄酮成分槐苷的含量及建立槐苷的含量测定标准。方法 :用系统分离法提取分离槐角得到异黄酮粗提物。与标准品对照 ,以PH2.73磷酸 -乙腈(72:28)作流动相 ,检测波长260nm ,用标准曲线方程法测定槐苷含量。结果 :测得槐角中槐苷占总异黄酮粗提物的27.9%,占总生药的2.233%,含量较高。所建立方法的加样回收率为97.4%,RSD0.85 %。结论 :该方法简便准确 ,重现性好。槐角中槐苷的含量较高 。Objective: A method for the quantification of Sophoricoside in Sophora japonica by HPLC was established. Methods: Using C18 column (10, YWG, ID4.0mm), Sophoricoside was separated and determined with mobile phase PH 2.7 phosphoric acid solution-acetonitride (72:28). The UV detection wavelength was 260nm. Results: The average revoveries of methods was 98.5% and the precision (RSD) was 0.7%(n=5). The content of sophoricoside in sophora japonica was 2.2% and 27.9% of all extract. Conclusion: The method was accurate, rapid and with good reproducibility. This method would be used in quality controlling of sophoricoside production and finding other sophoricoside resource.

关 键 词：中药 槐角 槐苷含量 HPLC 异黄酮 测定方法 反相高效液相色谱法 系统分离法 

分 类 号：R284.1[医药卫生—中药学] Q946.83[医药卫生—中医学]