康复训练对成年大鼠脑梗死后海马齿状回神经前体细胞增殖的影响  

Effect of rehabilitation training on the proliferation of the dentate gyrus neural precursor cells after cerebral infarction in adult rat

作　　者：王卫东[1] 王洪典[2] 王津存[2] 江文[2] 袁华[3] 李薇[3] 张志军[2] 

机构地区：[1]武警江西总队医院神经内科,南昌330001 [2]第四军医大学西京医院神经内科 [3]第四军医大学西京医院康复理疗科

出　　处：《中华物理医学与康复杂志》2003年第7期386-389,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的　研究康复训练对成年大鼠脑梗死后海马齿状回神经前体细胞增殖水平的影响。方法　采用光化学法诱导成年大鼠脑梗死 2 4h后 ,将大鼠随机分为梗死对照组、梗死后康复训练组和梗死后制动组。康复训练组每天进行水迷宫、转棒、滚笼训练 ,制动组于网状笼内固定。采用BrdU标记分裂细胞方法 ,观察、比较脑梗死后各组大鼠齿状回BrdU阳性细胞数量水平的变化规律和差异。结果　成年大鼠脑梗死后 6～2 4d ,梗死对照组大鼠齿状回BrdU阳性细胞数量显著增加 (P <0 .0 1) ,其中第 12天大鼠齿状回BrdU阳性细胞数量达到峰值。梗死后康复训练组各时间点大鼠海马齿状回BrdU阳性细胞数量较梗死对照组和梗死后制动组大鼠均明显增加 (P <0 .0 1) ,而梗死后制动组大鼠与梗死对照组大鼠齿状回BrdU阳性细胞数量水平则无显著性差异 (P >0 .0 5 )。结论　康复训练促进脑梗死后海马齿状回神经前体细胞增殖水平上调的作用可能是康复训练帮助脑梗死后神经功能恢复的一个重要机制。Objective To study the effects of rehabilitation training on the proliferation of the dentate gyrus neural precursor cells after cerebral infarction in the adult rat. Methods After the establishment of animal models of cerebral infarction, 75 Sprague-Dawley rats were divided into different groups randomly: the infarction control group, rehabilitation training group, and immobilization group. The rats in the rehabilitation group were given water maze, rotating bar and rolling cage exercises every day, while the rats in the immobilization group were fixed in cages. The number of neural precursor cells in the dentate gyrus were counted at 3, 6,12,24, and 48 days post-infarction, respectively, by using Bromodeoxyuridine (BrdU) labelling method, and comparisons were made among the rehabilitation training group, immobilization group and the infarction control group at various time points. Results In the infarction control group, the proliferation of the neural precursor cells in the dentate gyrus was enhanced in 6 to 24 days after cerebral infarction with its peak on the 12th day post-infarction (P<0.01). As compared with the immobilization group and infarction control group, the number of BrdU-labeled cells in the dentate gyrus was significantly increased after rehabilitation training on all the time points after infarction(P<0.01).However, no significant difference between the immobilization group and infarction control group was found(P>0.05). Conclusion The effect of rehabilitation training on the proliferation of neural precursor cells in the dentate gyrus may be one of the factors which promote the recovery of neural function after infarction in adult rats.

关 键 词：脑梗死 神经前体细胞 细胞增殖 康复训练 5-溴脱氧尿核苷 

分 类 号：R493[医药卫生—康复医学]