GENOMIC FEATURES OF COTESIA PLUTELLAE POLYDNAVIRUS  

小菜蛾绒茧蜂多分DNA病毒的基因组特征(英文)

作　　者：LIUCai-ling ZHUXiang-xiong FuWen-jun ZHAOMu-jun 

机构地区：[1]Shanghai Institute of Plant Physiology and Ecology,Shanghai Institute for Biological Sciences,the Chinese Academy of Sciences,Shanghai 200025,China [2]Shanghai Institute of Biochemistry and Cell Biology,Shanghai Institute for Biological Sciences,the Chinese Academy of Sciences,Shanghai 200031,China

出　　处：《Entomologia Sinica》2003年第2期103-108,共6页中国昆虫科学（英文版）

摘　　要：Polydnavirus was purified from the calyx fluid of Cotesia plutellae ovary. The genomic features of C. plutellae polydnavirus (CpPDV) were investigated. The viral genome consists of at least 12 different segments and the aggregate genome size is a lower estimate of 80kbp. By partial digestion of CpPDV DNA with BamHI and subsequent ligation with BamHI-cut plasmid Bluescript, a representative library of CpPDV genome was obtained.Polydnavirus was purified from the calyx fluid of Cotesia plutellae ovary. The genomic features of C. plutellae polydnavirus (CpPDV) were investigated. The viral genome consists of at least 12 different segments and the aggregate genome size is a lower estimate of 80kbp. By partial digestion of CpPDV DNA with BamHI and subsequent ligation with BamHI-cut plasmid Bluescript, a representative library of CpPDV genome was obtained.

关 键 词：Cotesia plutellae POLYDNAVIRUS GENOME genomic library 

分 类 号：Q963[生物学—昆虫学] Q78