益气活血法并高压氧对沙土鼠脑缺血重灌流脑膜微循环的作用  

Effects of supplementing Qi and activating blood circulation and hyperbaric oxygenation on meninges microcirculation in gerbil after cerebral ischemic reperfusion injury

作　　者：俞国平[1] 方敏[1] 

机构地区：[1]上海市闸北区中心医院,上海市200070

出　　处：《中国临床康复》2003年第19期2650-2651,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的探讨中医益气活血法并高压氧对沙土鼠脑缺血重灌流后脑膜微循环的作用。方法用LMB-1激光显微血流计及其电视录像系统,通过颅窗量化观察4组蒙古种沙土鼠(每组各11只)颈总动脉阻断前、阻断后重灌流以及4种方案处理后120min内软脑膜微循环的变化。结果注射益气活血药及高压氧治疗后,脑缺血动物脑膜微循环血供明显增加,血流速度加快,细动脉流速达1.46～1.56mm/s,细静脉流速达1.27～1.44mm/s,与对照组相比,P<0.05～0.01,脑膜微血栓凝块逐渐疏松,微血管周围渗出明显减轻甚至消退。结论益气活血法并高压氧对沙土鼠脑缺血重灌流后脑膜微循环的作用确切,其直接作用与加快血流速度,调控微血管舒缩功能,改善大脑血供等有关,两者合用作用互补,疗效显著。Aim To explore the efficacy of Chinese drugs with the function of supplementing Qi and activating blood circulation and hyperbaric oxygen(HBO) on the meningeal microcirculation in gerbil after cerebral ischemia/reperfusion injury.Methods Forty four Mongolian gerbils underwent following operations:a cranial window was created in the left parietal bone and covered with absorbent cotton soaked with artificial cerebro spinal fluid.The bilateral common carotid arteries were squeezed by clamps for 30 minutes and then the clamps were loosened so as to cause reperfusion. The gerbils were equally divided into four groups at random:drug group (compound solution including astragalus root,Chinese angelica,and red sage root was infused intraperitoneally),HBO group(exposed to 0.25 MPa hyperbaric oxygen for 60 min),combined treatment group (both the two above methods were used),and control group(normal saline was infused intraperitoneally).LMB 1 laser microcirculatory meter and JI 200 laser microcirculatory moving analytical meter were used to observe through the cranial windows the changes of meningeal microcirculation before cerebral ischemia, after cerebral ischemia and reperfusion for 120 min in these 4 groups. Results After the common carotid arteries were squeezed,the microvessels of the meninges contracted obviously, microthrombus were seen,and blood flow rate of the arteriole decreased to 0.45 mm/s,and the blood flow rate of the venules decreased to 0.38 mm/s.It was observed that in the drug group, HBO group,and combined treatment group the microthrombi in meninges were loosened gradually,and hydrops around microvessel was gradually relieved up to extinction.The arteriole blood flow rate was 1.46- 1.56 mm/s,the venule blood flow rate was 1.27- 1.44 mm/s, significantly higher than those of the control group (P< 0.05- 0.01).Conclusion Hyperbaric oxygenation and traditional Chinese drugs with the function of supplementing Qi and activating blood circulation play a role in improving microcirculation.Their ef

关 键 词：益气活血法 高压氧 沙土鼠 脑缺血 脑膜微循环 补阳还五汤 再灌注损伤 

分 类 号：R277.733.1[医药卫生—中医学]