SEPSA法在反复输血患者血小板同种抗体检查中的应用  

Application of SEPSA Method in Detection of Platlet Isotype Antibody on Repeated Transfusion

作　　者：张易水 张晓明[1] 裴丽萍[1] 

机构地区：[1]佳木斯市红十字中心血站,黑龙江佳木斯154000

出　　处：《黑龙江医学》2003年第8期585-586,共2页Heilongjiang Medical Journal

摘　　要：目的　反复输全血或血小板制品可导致同种血小板抗体增加 ,进而造成输血效果欠佳等相关副反应 ,利用SEPSA法可筛查此类患者的血小板抗体。方法　采用上海血流中心提供的SEPSA技术ELA淋巴细胞毒试验。HLA和血小板特异性抗体鉴别按SEPSA法做磷管氯喹处理。结果　多次输血及浓缩血小板造成同种血小板抗体升高达 4 6 72 %。SEPSA法对此类患者检查血小板抗体灵敏度高。Objective To discuss the screen detection of platelet isotype antibody with SEPSA method due to antibody increasing that lead the relative side effects because of repeated transfusion and platelet product. Methods The HLA and platelet specific antibody were differentiated with chloroquine diphosphate accoding to ELA lymphocyte toxicity test supplied by Shanghai Blood Center. Results The isotype platelet antibody was 46.72% in repeated transfusion and concentrated platelet transfusion. SEPSA could detect the antibody. Conclusion SEPSA method should be efficacy in screening platelet antibody.

关 键 词：临床诊断学 反复输血 血小板同种抗体 SEPSA法 ELA淋巴细胞毒试验 

分 类 号：R457.1[医药卫生—治疗学]