胚胎干细胞来源的BL-CFC可产生高增殖潜能造血祖细胞  

Generation of High Proliferative Potential Hematopoietic Progenitor Cells from Embryonic Stem Cell-Derived BL-CFC

作　　者：要晖宇[1] 刘兵[1] 原野[1] 毛宁[1] 

机构地区：[1]军事医学科学院基础医学研究所细胞生物学研究室,北京100850

出　　处：《中国实验血液学杂志》2003年第4期345-349,共5页Journal of Experimental Hematology

基　　金：国家高技术研究发展计划"863"资助项目 (编号2 0 0 1AA2 1713 1);国家重点基础研究发展规划"973"资助项目 (编号G19990 5 43 0 2 )

摘　　要：小鼠胚胎干细胞 (embryonicstemcell,EScell)体外分化 2 .5 - 3.5天形成的拟胚体 (embryoidbody ,EB)中可产生原始细胞集落形成细胞 (blastcolony formingcell,BL CFC) ,后者具有造血和内皮细胞双向分化潜能 ,是目前体外实验可检测到的最早期的定向造血分化的细胞。本研究建立BL CFC培养体系 ,并通过造血祖细胞集落培养、免疫荧光标记以及巢式RT PCR鉴定单个原始细胞集落来源的贴壁细胞和非贴壁细胞的分化特点。结果表明 :部分贴壁细胞吞噬DiI Ac LDL ,并表达CD31,UEA I ,VE cadherin等内皮细胞特异性的表面分子 ;非贴壁细胞具有原始造血 (primitivehematopoiesis)和 (或 )永久造血 (definitivehematopoiesis)活性 ,其中 2 0 %的原始细胞集落含有高增殖潜能集落形成细胞 (highproliferativepotentialcolony formingcell,HPP CFC) ,后者能形成次级造血集落。结论 :胚胎干细胞来源的BL CFC可产生高增殖潜能造血祖细胞 ,但原始细胞集落来源的HPPThe blast colony forming cells (BL CFC), which are detected within embryoid bodies derived from embryonic stem cells (ES cells) differentiated for 2.5-3.5 days, have dual potential of differentiation to hematopoietic and endothelial cells. In this investigation the culture method of BL CFC was established and colony forming assay, immunofluorescent technique as well as nested RT PCR was employed to identify the differentiation capacity of adherent and nonadherent cells derived from individual blast colony. The results showed that the adherent cells could intake DiI Ac LDL and expressed the endothelium specific surface markers including CD31,UEA I and VE cadherin. In addition, nonadherent cells were capable of developing primitive or/and definitive hematopoiesis potential. High proliferative potential colony forming cells (HPP CFC) bearing self renewal capacity was found in 20% of BL CFC. It is concluded that BL CFC derived from embryonic stem cells can generate high proliferative potential hematopoietic progenitor cells. However, the whether BL CFC can reconstitute the adult bone marrow hematopoiesis in long term remains to be further determined.

关 键 词：胚胎干细胞 原始集落形成细胞 高增殖潜能集落形成细胞 造血分化 

分 类 号：R331.2[医药卫生—人体生理学]