氯离子通道阻断剂对肺血管内皮细胞氧化损伤的保护作用  

The protective effects of chloride channel blockers on pulmonary artery endothelial cell injury induced by oxidant

作　　者：薛军[1] 张丙芳[1] 贾敏[2] 招明高[2] 晏伟[3] 付勇[3] 徐宏勇[3] 梅其炳[2] 戚好文[1] 

机构地区：[1]解放军第四军医大学西京医院呼吸内科,陕西省西安市710033 [2]解放军第四军医大学基础部药理学教研室,陕西省西安市710033 [3]解放军第四军医大学基础部药理学教研室基础部病理学教研室,陕西省西安市710033

出　　处：《中国临床康复》2003年第21期2906-2907,F003,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:研究氯离子通道阻断剂在肺血管内皮细胞氧化损伤中的作用。方法:以肉联厂检疫合格的健康小牛为研究对象,过氧化氢(H2O2)诱导体外培养的牛肺动脉内皮细胞损伤,观察Cl-通道阻断剂对受损细胞细胞活力、乳酸脱氢酶(LDH)释放量、三磷酸腺苷(ATP)含量、细胞内钙离子浓度犤Ca2+犦i和DNA降解程度的影响。结果:Cl-通道阻断剂可使受损细胞的细胞活力得到提高,NPPB组和NFA组分别恢复到0.449±0.015和0.535±0.023;LDH释放量下降,分别为(12.4±0.4)%,(6.4±0.6)%。ATP含量分别回升为18.22nmol/mg蛋白质和21.96nmol/mg蛋白质,犤Ca2+犦i下降和DNA降解程度减轻。结论:Cl-通道参与了氧化剂对肺血管内皮细胞损伤的病理过程,Cl-通道阻断剂对肺血管内皮细胞的损伤具有保护作用。AIM:To study the effect of chloride (Cl- ) channel blockers on pulmonary artery endothelial cell injury induced by oxidant H2O2. METHODS:Cultured pulmonary artery endothelial cells obtained from health calf (CAPE cells) exposed to H2O2 were used to observe the effect of Cl- channel blocker stress on cell vitality, lactate dehydrogenase (LDH) release, adenosine triphosphate (ATP) contents,i and DNA damage.RESULTS:The Cl- channel blockers (NPPB and NFA) markedly promoted cell vitality, which in NPPB group was promoted to 0.449± 0.015, and in NFA group was 0.535± 0.023. The release of LDH of part NPPB was reduced to (12.4± 0.4)% ,and that of part NFA was (6.4± 0.6)% . The ATP content was increased to 18.22 and 21.96 nmol/mg respectively, with the content of i and the DNA fragmentation of damaged cells decreased.

关 键 词：氯离子通道阻断剂 肺血管内皮细胞 肺细胞氧化损伤 乳酸脱氢酶 三磷酸腺苷 肺细胞保护 

分 类 号：R972[医药卫生—药品] R329.2[医药卫生—药学]