菊延保康冲剂对吗啡依赖大鼠戒断治疗的作用评价  

Therapeutic evaluation of" Ju Yan Bao Kang Keli" in detoxication therapy for morphine dependent rats

作　　者：陆贤杰[1] 覃园程[1] 潘励山 梁峰[1] 方文贞[1] 

机构地区：[1]右江民族医学院药物依赖研究所,广西壮族自治区百色市533000 [2]中国药物依赖研究所毒理研究室,北京市100083

出　　处：《中国临床康复》2003年第21期2930-2931,共2页Chinese Journal of Clinical Rehabilitation

基　　金：广西壮族自治区教育厅资助课题(00403028);卫生部批准立项研制~~

摘　　要：目的:观察菊延保康冲剂对吗啡依赖大鼠戒断综合征的控制效果,旨在为戒毒康复研究提供药效学依据。方法:Wister种大鼠100只,雄雌兼用,体质量180～220g。以剂量递增法形成大鼠吗啡依赖模型,其中50只于第15天做催促戒断试验,随机分为阴性(NS)对照组、阳性对照组(可乐定,0.2mg/kg,灌胃),菊延保康冲剂Ⅰ、Ⅱ、Ⅲ组(以每kg体质量1.4g,2.4g,4.0g灌胃)后用纳洛酮(4mg/kg)催促,记录各组1h内大鼠的戒断反应。另50只大鼠于第29天早上做自然戒断试验,分成5组,每组10只,阴性(NS)对照组给予等容量生理盐水3次/d灌胃,阳性对照组(给予可乐定1mg/kg灌胃),菊延保康冲剂Ⅰ、Ⅱ、Ⅲ组分别给予每次0.7g/kg,1.4g/kg,2.4g/kg灌胃,给药连续1周。治疗前1d及治疗期间每天给药前称大鼠体质量。结果:菊延保康冲剂3个剂量组的戒断症状分值小于NS组,差异有非常显著性意义(P<0.01)。戒断症状分值大于可乐定组,但大、中剂量组与可乐定组比较差异无显著性意义(P>0.05)。菊延保康冲剂3个剂量组的体质量下降百分率低于NS组和可乐定组。吗啡依赖大鼠自然戒断试验结果显示菊延保康冲剂小剂量和中剂量组在治疗前3d对体质量下降均有显著的控制作用(P<0.05～P<0.01)。大剂量组从第3天开始体质量没有恢复,并继续下降。可乐定组完全不能控制吗啡依?AIM:To observe the therapeutic efficacy of ' Ju Yan Bao Kang Keli' (BKKL) in inhibition of the withdrawal syndrome in morphine dependent rats and to provide pharmacological basis for study of detoxicate rehabilitation.METHODS:One hundred Wistar rats,weighing 180- 220 g, were enrolled in this study.The dependence model was established by gradually increasing doses of morphine. Morphine was injected for 15 days,and 50 rats were subjected to press withdrawal experiment, and randomly divided into 5 groups:negative control group(normal saline,NS),positive control group(clonidine, 0.2 mg/kg, i.g.) and 3 experimental groups of Ⅰ ,Ⅱ and Ⅲ (BKKL,1.4 g/kg group, 2.4 g/kg group and 4.0 g/kg group, i.g.). Two hours after administration, pressed by Naloxone(4 mg/kg),and different reaction of abstinent syndromes were observed and recorded with 1 h.Other 50 Wistar rats were employed in natural withdrawal experiment and randomly divided into 5 groups of 10 cases each at the 29th day: negative control group (NS i.g.), positive control group(clonidine, 0.1 mmg/kg, i.g.) and 3 experiment groups of Ⅰ ,Ⅱ and Ⅲ (BKKL, 0.7 g/kg,1.4 g/kg and 2.4 g/kg, i.g.). The duration of administration was 1 week. Body mass index (BMI) was measured at 1 d before treatment and during the treatment. RESULTS: The scores of withdrawal syndromes of the three experimental groups were lower than that of the negative group in the pressed withdrawal experiment, with a significant difference(P< 0.01). However, the scores of withdrawal syndromes of the three experimental groups was higher than that of the positive control group, but there was no significant difference between the low and middle dose groups and clonidine group(P >0.05). Comparing the percentage of reduced BMI in natural withdrawal experiment, BMI of three experimental groups was lower than that of the negative and positive control groups. Natural withdrawal experiment showed that 'Bao Kang Keli' (0.7 g/kg group and 1.4 g/kg group) significantly inhibited the weight of morphine abs

关 键 词：菊延保康冲剂 吗啡依赖 大鼠 治疗 戒断综合征 可乐定 

分 类 号：R259.955[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]