细胞凋亡与糖尿病肾病的关系及糖肾康作用机制的研究  

The Researches on the Relation of Apoptosis with Diabetic Nephropathy and the Mechanism of Tangshenkang

作　　者：赵玲[1] 顾江萍[2] 栗德林[3] 

机构地区：[1]广州中医药大学第二临床医学院,广州510120 [2]辽宁省大连市第二人民医院,大连116011 [3]黑龙江中医药大学,哈尔滨150041

出　　处：《中国中西医结合肾病杂志》2003年第8期439-441,共3页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的:从细胞凋亡角度探讨糖尿病肾病(DN)的发病机理及糖肾康对糖尿病肾病细胞凋亡的影响。方法:将60只大鼠随机分为5组:空白对照组、模型组、中药预防组(糖肾康8.75g·kg^(-1)·d^(-1))、中药高剂量组(糖肾康17.5g·kg^(-1)·d^(-1))中药低剂量组(糖肾康8.75g·kg^(-1)·d^(-1))。糖尿病肾病大鼠模型以小剂量链脲佐菌素腹腔注射配合高糖饮食制作;治疗6周后,采用原位末端标记法和免疫组化方法检测肾组织内细胞凋亡及Fas/FasL、Bcl-2等相关基因的表达。结果:模型组大鼠肾组织内细胞凋亡明显增多,Fas/FasL基因表达增加,Bcl-2基因表达减少,与其他组别相比有统计学差异(P<0.05或P<0.01);模型组PKC主要表达在肾小球和肾小管的细胞膜上,而其他组则主要表达于胞浆。结论:糖尿病肾病大鼠肾组织尤其是肾小管细胞凋亡明显增多,糖肾康可通过降低Fas/FasL基因表达,促进Bcl-2基因表达,抑制PKC在胞膜的表达而起到控制肾组织内细胞凋亡的作用。Objective: To investigated the mechanism of diabetic nephropathy (DN) and the influence of Tang-shenkang (TSK) to apoptosis. Methods: 60 Wistar rats were randomly divided into 5 groups:normal contrast group, pattern group, prevention group ,17.5 g · kg -1 · d-1 TSK group ,8.75 g · kg -1 · d -1 TSK group. The experimental rats model of DN were reconstructed by inject lower dosage STZ into the rat's abdominal cavity and fed in higher quantity of heat. Water and drug were delivered by gavage for 6 weeks. We detected apotosis and the gene expresses of Fas/FasL, Bcl - 2 and PKC of renal tissues with the methods of terminal label in situ and immunohistochemistry. Results: Compared with other groups, the apototic cells were more in number, the gene expression of Fas/FasL were increasing and the gene expression of Bcl - 2 were decreasing in the pattern group(P<0.05orP<0.01).The locations of the expression of PKC in pattern group were mainly in the cell membrane of renal glomerulus and renal tubules,other groups were mainly expressed in the cell plasm. Conclusion: In the renal tissues especialy renal tubules of the DN rats, the numbers of apoptotic cells increased obviously, it was regulated by Fas/FasL,and influenced by Bcl - 2 and PKC. TSK could control apoptosis of the renal tissues by influenced the gene express of Fas/FasL,Bcl - 2 and PKC.

关 键 词：细胞凋亡 糖尿病肾病 糖肾康 大鼠 免疫组织化学 中药 

分 类 号：R277.5[医药卫生—中医学]