Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes  

作　　者：YINGCHEN ZHIXuHE AILIANLIU KAIWANG WENWEIMAO JIANKINCHU YONGLU ZHENGFUFANG YINGTANGSHI QINGZHANGYANG DAYUANCHEN MINKANGWANG JINSONGLI SHAOLIANGHUANG XIANGYINKONG YAOZHOUSHI ZHIQIANGWANG JIAHuIXIA ZHIGAOLONG ZHIGANGXUE WENXIANGDING HUIZHENSHENG 

机构地区：[1]CenterforDevelopmentalBiology,ShanghaiSecondMedicalUniversity,Shanghai200092,China [2]CenterforStemCellResearch,theSecondAffiliatedHospital,SunYat-SenUniversity,Guangzhou510120,China [3]LaboratoryofStemCellBiology,-instituteofBiochemistryandCellBiology,ShanghaiInstitutesforBio-logicalSciences,ChineseAcademyofSciences,1665KongJiangRoad,Shanghai200092,China [4]StateKeyLaboratoryofReproductiveBiology,InstituteofZoology,ChineseAcademyofSciences,19Zhong-guanCunRoad,HaidianDistrict,Beijing100080,China [5]HealthScienceCenter,ShanghaiInstitutesofBiologicalSciences,ChineseAcademyofSciencesandShang-haiSecondMedicalUniversity,Shanghai200025,China [6]NationalLaboratoryofMedicalGenetics,CentralSouthUniversity,88XiangyaRoad,Changsha410078,China [7]XinhuaHospital,ShanghaiSecondMedicalUniversity,1665KongJiangRoad,Shanghai200092,China

出　　处：《Cell Research》2003年第4期251-263,共13页细胞研究（英文版）

基　　金：supported by grants from the Major State Basic Research Development Program of China(No.001CB5099);the National High Technology Research and Development Program of China(No.2001AA216121);National Natural Science Foundation of China(No.30040003);Projects of Shanghai Science&Technology Development Foundation(No.99DJ14002,00DJ14033,01DJ14003);the Chinese Academy of Sciences(No.KSCX-2-3-08);Shanghai Municipal Education Commission and by Shanghai Second Medical University

摘　　要：To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PCR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well as mixed cell populations that express markers representative of all three germ layers. Thus, ntES cells derived from human somatic cells by NT to rabbit eggs retain phenotypes similar to those of conventional human ES cells, including the ability to undergo multilineage cellular differentiation.

关 键 词：nuclear transfer (NT) somatic cell nuclear transfer (SCNT) embryonic stem cells (ES cell) therapeutic cloning rabbit oocyte. 

分 类 号：Q813.3[生物学—生物工程]