杉木雄性不育株与可育株小孢子叶球的发育解剖和生理生化的比较研究  

COMPARATIVE STUDIES ON DEVELOPMENTAL ANATOMY, PHYSIOLOGY AND BIOCHEMISTRY OF MICROSTROBILUS BETWEEN MALE STERILE AND FERTILE PLANTS IN CUNNINGHAMIA LANCEOLATA

作　　者：李平[1] 余象煜[1] 吕洪飞[1] 

机构地区：[1]杭州大学生物系

出　　处：《林业科学》1992年第2期115-121,共7页Scientia Silvae Sinicae

基　　金：浙江省自然科学基金;浙江省教委基金

摘　　要：1978年我们在浙江省首次发现杉木(Cunnighamia lanceolata)雄性不育株。本文对杉木雄性不育株的小孢子叶球进行了有关形态学和生理生化方面的初步研究。结果表明:杉木的雄性不育属于无孢子型。造成雄性不育的直接原因是绒毡层的过早解体和小孢子母细胞不能完成减数分裂。雄性不育株的雌球花发育正常,并能正常开花、授粉和结籽。在小孢子发生和发育过程中,不育株小孢子叶球的呼吸强度明显低于可育株的小孢子叶球,并表现出一定的规律性。不育株与可育株之间过氧化物酶同工酶谱具有明显差异,前者的酶带数始终多于后者。作者认为,杉木不育株中可能存在雄性不育细胞质基因,通过影响或调节编码过氧化物酶的核基因的表达,或影响核基因遗传功能的正常发挥,引起核质之间的不协调而导致雄性不育。The male sterile plant of C. lanceolata Hook. was found by us in Zhejiang province in 1978. This paper descfibes a seties of studies on the morphology, physiology and biochemistry of the microstrobilus of the male sterile plant of C. lanceolata. The male sterility of C. lanceolata belongs to the pollen-free type. The direct causes of the male sterility of C. lanceolata may be that the tapetum disintegrates in advance and that the microspore mother cells call not finish meiosis. The female cone of the male sterile plant can blossom, pollinate and bear seeds normally. During the genesis and development of the microspore, the respiratory intensity of the microstrobilus in the male sterile plant is obviously lower than that of the microstrobilus in the male fertile plant. There is a significant difference in the charts of peroxidase isozyme between the male sterile and fertile plants. It shows that the bands of the former are always more than that of the latter It is suggested that there exists the male sterile cytopleam gene in the male sterile plants, which can influemces the normal function or/and regulates the expression of nucleal, genes that coded the peroxidase isozymes, and the discordance between nuclear gene and cytopleam gene can result in the male sterility

关 键 词：杉木 小孢子叶球 雄性不育 

分 类 号：S791.270.4[农业科学—林木遗传育种]