VP7/IFNα-2b融合蛋白防治轮状病毒腹泻的可行性  

Possibility of a fusion protein VP7/IFNα-2b on preventing and treating rotavirus diarrhoea

作　　者：张泰芳[1] 周鹏[1] 

机构地区：[1]华南热带农业大学热带作物生物技术国家重点实验室，海口571101

出　　处：《生物技术通讯》2003年第4期336-338,共3页Letters in Biotechnology

基　　金：国家自然科学基金资助项目(30060033)

摘　　要：轮状病毒(RV)外壳蛋白VP7可用作抗原;通过构建植物表达载体,将该抗原基因导入植物细胞,最终在植物细胞中获得抗原蛋白的表达;这种蛋白能保持自然免疫特性,口服能诱发粘膜免疫反应,从而达到对RV的预防作用。干扰素作为一种广谱的抗病毒蛋白质,能对RV引起的肠炎起治疗作用。本文提出利用干扰素与轮状病毒表面抗原融合蛋白用于防治轮状病毒腹泻的构想,并对其可行性作了探讨。Outer capsid structural protein VP7of rotavirus can be used as antigen,which gene would be integrated into plant cell by constructing plant expression vector and be expressed in the plant cell.The vaccine with native immunongenic properties would be produced by transgenic plants.The edible vaccines can stimulate mucosal im-munonresponses so that RV can be taken precautions against effectively.Interferon is a kind of antiviral protein substances,which can treat RV enterities.We present a kind of assumption that interferon and rotavirus surface antigen can be fused to prevent and treat rotavirus diarrhoea.There is a discussion about feasibility in this article.

关 键 词：融合蛋白 防治 轮状病毒 腹泻 口服疫苗 干扰素 

分 类 号：R373.2[医药卫生—病原生物学] Q78[医药卫生—基础医学]